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Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()

Seminal vesicle secretions (SVSs), together with spermatozoa, are ejaculated into the female reproductive tract. SVS7, also known as PATE4, is one of the major SVS proteins found in the seminal vesicle, copulatory plug, and uterine fluid after copulation. Here, we generated Pate4 knockout (−/−) mice...

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Autores principales: Noda, Taichi, Fujihara, Yoshitaka, Matsumura, Takafumi, Oura, Seiya, Kobayashi, Sumire, Ikawa, Masahito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483057/
https://www.ncbi.nlm.nih.gov/pubmed/30452524
http://dx.doi.org/10.1093/biolre/ioy247
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author Noda, Taichi
Fujihara, Yoshitaka
Matsumura, Takafumi
Oura, Seiya
Kobayashi, Sumire
Ikawa, Masahito
author_facet Noda, Taichi
Fujihara, Yoshitaka
Matsumura, Takafumi
Oura, Seiya
Kobayashi, Sumire
Ikawa, Masahito
author_sort Noda, Taichi
collection PubMed
description Seminal vesicle secretions (SVSs), together with spermatozoa, are ejaculated into the female reproductive tract. SVS7, also known as PATE4, is one of the major SVS proteins found in the seminal vesicle, copulatory plug, and uterine fluid after copulation. Here, we generated Pate4 knockout (−/−) mice and examined the detailed function of PATE4 on male fecundity. The morphology and weight of Pate4−/− seminal vesicles were comparable to the control. Although Pate4−/− cauda epididymal spermatozoa have no overt defects during in vitro fertilization, Pate4−/− males were subfertile. We found that the copulatory plugs were smaller in the vagina of females mated with Pate4−/− males, leading to semen leakage and a decreased sperm count in the uterus. When the females mated with Pate4−/− males were immediately re-caged with Pate4+/+ males, the females had subsequent productive matings. When the cauda epididymal spermatozoa were injected into the uterus and plugged artificially [artificial insemination (AI)], Pate4−/− spermatozoa could efficiently fertilize eggs as compared to wild-type spermatozoa. We finally examined the effect of SVSs on AI, and observed no difference in fertilization rates between Pate4+/+ and Pate4−/− SVSs. In conclusion, PATE4 is a novel factor in forming the copulatory plug that inhibits sequential matings and maintains spermatozoa in the uterus to ensure male fecundity.
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spelling pubmed-64830572019-04-30 Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity() Noda, Taichi Fujihara, Yoshitaka Matsumura, Takafumi Oura, Seiya Kobayashi, Sumire Ikawa, Masahito Biol Reprod Research Article Seminal vesicle secretions (SVSs), together with spermatozoa, are ejaculated into the female reproductive tract. SVS7, also known as PATE4, is one of the major SVS proteins found in the seminal vesicle, copulatory plug, and uterine fluid after copulation. Here, we generated Pate4 knockout (−/−) mice and examined the detailed function of PATE4 on male fecundity. The morphology and weight of Pate4−/− seminal vesicles were comparable to the control. Although Pate4−/− cauda epididymal spermatozoa have no overt defects during in vitro fertilization, Pate4−/− males were subfertile. We found that the copulatory plugs were smaller in the vagina of females mated with Pate4−/− males, leading to semen leakage and a decreased sperm count in the uterus. When the females mated with Pate4−/− males were immediately re-caged with Pate4+/+ males, the females had subsequent productive matings. When the cauda epididymal spermatozoa were injected into the uterus and plugged artificially [artificial insemination (AI)], Pate4−/− spermatozoa could efficiently fertilize eggs as compared to wild-type spermatozoa. We finally examined the effect of SVSs on AI, and observed no difference in fertilization rates between Pate4+/+ and Pate4−/− SVSs. In conclusion, PATE4 is a novel factor in forming the copulatory plug that inhibits sequential matings and maintains spermatozoa in the uterus to ensure male fecundity. Oxford University Press 2019-04 2018-11-18 /pmc/articles/PMC6483057/ /pubmed/30452524 http://dx.doi.org/10.1093/biolre/ioy247 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Noda, Taichi
Fujihara, Yoshitaka
Matsumura, Takafumi
Oura, Seiya
Kobayashi, Sumire
Ikawa, Masahito
Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title_full Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title_fullStr Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title_full_unstemmed Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title_short Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
title_sort seminal vesicle secretory protein 7, pate4, is not required for sperm function but for copulatory plug formation to ensure fecundity()
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483057/
https://www.ncbi.nlm.nih.gov/pubmed/30452524
http://dx.doi.org/10.1093/biolre/ioy247
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