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Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483087/ https://www.ncbi.nlm.nih.gov/pubmed/30205273 http://dx.doi.org/10.1016/j.virol.2018.08.022 |
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author | O’Brien, Amornrat Mettelman, Robert C. Volk, Aaron André, Nicole M. Whittaker, Gary R. Baker, Susan C. |
author_facet | O’Brien, Amornrat Mettelman, Robert C. Volk, Aaron André, Nicole M. Whittaker, Gary R. Baker, Susan C. |
author_sort | O’Brien, Amornrat |
collection | PubMed |
description | Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>10(7) pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20 h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC. |
format | Online Article Text |
id | pubmed-6483087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64830872019-12-01 Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines O’Brien, Amornrat Mettelman, Robert C. Volk, Aaron André, Nicole M. Whittaker, Gary R. Baker, Susan C. Virology Article Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>10(7) pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20 h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC. Elsevier Inc. 2018-12 2018-09-08 /pmc/articles/PMC6483087/ /pubmed/30205273 http://dx.doi.org/10.1016/j.virol.2018.08.022 Text en © 2018 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article O’Brien, Amornrat Mettelman, Robert C. Volk, Aaron André, Nicole M. Whittaker, Gary R. Baker, Susan C. Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title | Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title_full | Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title_fullStr | Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title_full_unstemmed | Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title_short | Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines |
title_sort | characterizing replication kinetics and plaque production of type i feline infectious peritonitis virus in three feline cell lines |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483087/ https://www.ncbi.nlm.nih.gov/pubmed/30205273 http://dx.doi.org/10.1016/j.virol.2018.08.022 |
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