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Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines

Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here...

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Autores principales: O’Brien, Amornrat, Mettelman, Robert C., Volk, Aaron, André, Nicole M., Whittaker, Gary R., Baker, Susan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483087/
https://www.ncbi.nlm.nih.gov/pubmed/30205273
http://dx.doi.org/10.1016/j.virol.2018.08.022
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author O’Brien, Amornrat
Mettelman, Robert C.
Volk, Aaron
André, Nicole M.
Whittaker, Gary R.
Baker, Susan C.
author_facet O’Brien, Amornrat
Mettelman, Robert C.
Volk, Aaron
André, Nicole M.
Whittaker, Gary R.
Baker, Susan C.
author_sort O’Brien, Amornrat
collection PubMed
description Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>10(7) pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20 h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC.
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spelling pubmed-64830872019-12-01 Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines O’Brien, Amornrat Mettelman, Robert C. Volk, Aaron André, Nicole M. Whittaker, Gary R. Baker, Susan C. Virology Article Investigating type I feline coronaviruses (FCoVs) in tissue culture is critical for understanding the basic virology, pathogenesis, and virus-host interactome of these important veterinary pathogens. This has been a perennial challenge as type I FCoV strains do not easily adapt to cell culture. Here we characterize replication kinetics and plaque formation of a model type I strain FIPV Black in Fcwf-4 cells established at Cornell University (Fcwf-4 CU). We determined that maximum virus titers (>10(7) pfu/mL) were recoverable from infected Fcwf-4 CU cell-free supernatant at 20 h post-infection. Type I FIPV Black and both biotypes of type II FCoV formed uniform and enumerable plaques on Fcwf-4 CU cells. Therefore, these cells were employable in a standardized plaque assay. Finally, we determined that the Fcwf-4 CU cells were morphologically distinct from feline bone marrow-derived macrophages and were less sensitive to exogenous type I interferon than were Fcwf-4 cells purchased from ATCC. Elsevier Inc. 2018-12 2018-09-08 /pmc/articles/PMC6483087/ /pubmed/30205273 http://dx.doi.org/10.1016/j.virol.2018.08.022 Text en © 2018 Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
O’Brien, Amornrat
Mettelman, Robert C.
Volk, Aaron
André, Nicole M.
Whittaker, Gary R.
Baker, Susan C.
Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title_full Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title_fullStr Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title_full_unstemmed Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title_short Characterizing replication kinetics and plaque production of type I feline infectious peritonitis virus in three feline cell lines
title_sort characterizing replication kinetics and plaque production of type i feline infectious peritonitis virus in three feline cell lines
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483087/
https://www.ncbi.nlm.nih.gov/pubmed/30205273
http://dx.doi.org/10.1016/j.virol.2018.08.022
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