Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method

The bone-specific alkaline phosphatase (ALP) isoenzyme activity was measured in 51 serum samples from four captive Asian elephants (Elephas maximus) using a conventional method with wheat germ lectin precipitation and a commercial agarose gel electrophoresis (AGE) kit; the isoenzymes were designated...

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Autores principales: TAKEHANA, Kazuya, ONOMI, Rurika, HATATE, Kaoru, YAMAGISHI, Norio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2019
Materias:
Wildlife Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483906/
https://www.ncbi.nlm.nih.gov/pubmed/30787213
http://dx.doi.org/10.1292/jvms.18-0688
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author TAKEHANA, Kazuya
ONOMI, Rurika
HATATE, Kaoru
YAMAGISHI, Norio
author_facet TAKEHANA, Kazuya
ONOMI, Rurika
HATATE, Kaoru
YAMAGISHI, Norio
author_sort TAKEHANA, Kazuya
collection PubMed
description The bone-specific alkaline phosphatase (ALP) isoenzyme activity was measured in 51 serum samples from four captive Asian elephants (Elephas maximus) using a conventional method with wheat germ lectin precipitation and a commercial agarose gel electrophoresis (AGE) kit; the isoenzymes were designated as bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3), respectively. This study examined the suitability of the AGE kit for analyzing blood biochemistry in Asian elephants. The serum ALP3 and BAP activities were strongly positively correlated and met the evaluation criteria for agreement using Bland-Altman analysis. The results indicate that the AGE kit can be used to examine the blood biochemistry in Asian elephants instead of the conventional method.
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spelling pubmed-64839062019-04-30 Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method TAKEHANA, Kazuya ONOMI, Rurika HATATE, Kaoru YAMAGISHI, Norio J Vet Med Sci Wildlife Science The bone-specific alkaline phosphatase (ALP) isoenzyme activity was measured in 51 serum samples from four captive Asian elephants (Elephas maximus) using a conventional method with wheat germ lectin precipitation and a commercial agarose gel electrophoresis (AGE) kit; the isoenzymes were designated as bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3), respectively. This study examined the suitability of the AGE kit for analyzing blood biochemistry in Asian elephants. The serum ALP3 and BAP activities were strongly positively correlated and met the evaluation criteria for agreement using Bland-Altman analysis. The results indicate that the AGE kit can be used to examine the blood biochemistry in Asian elephants instead of the conventional method. The Japanese Society of Veterinary Science 2019-02-21 2019-04 /pmc/articles/PMC6483906/ /pubmed/30787213 http://dx.doi.org/10.1292/jvms.18-0688 Text en ©2019 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Wildlife Science
TAKEHANA, Kazuya
ONOMI, Rurika
HATATE, Kaoru
YAMAGISHI, Norio
Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title_full Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title_fullStr Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title_full_unstemmed Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title_short Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method
title_sort determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive asian elephants (elephas maximus) using an agarose gel electrophoresis method
topic Wildlife Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6483906/
https://www.ncbi.nlm.nih.gov/pubmed/30787213
http://dx.doi.org/10.1292/jvms.18-0688
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