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A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza

Genistein is known to have multiple biological activities and has great potential for use as a preventative medicine and in disease treatment. Genistein can be extracted from plants, but also can be obtained from its glycoside form, sophoricoside, which is more abundant in some plants. Biotransforma...

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Autores principales: Mei, Jianfeng, Chen, Xiang, Liu, Jianghua, Yi, Yu, Zhang, Yanlu, Ying, Guoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484079/
https://www.ncbi.nlm.nih.gov/pubmed/31024087
http://dx.doi.org/10.1038/s41598-019-42996-z
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author Mei, Jianfeng
Chen, Xiang
Liu, Jianghua
Yi, Yu
Zhang, Yanlu
Ying, Guoqing
author_facet Mei, Jianfeng
Chen, Xiang
Liu, Jianghua
Yi, Yu
Zhang, Yanlu
Ying, Guoqing
author_sort Mei, Jianfeng
collection PubMed
description Genistein is known to have multiple biological activities and has great potential for use as a preventative medicine and in disease treatment. Genistein can be extracted from plants, but also can be obtained from its glycoside form, sophoricoside, which is more abundant in some plants. Biotransformation by unpurified microbial enzymes has the advantage of low cost and is a preferred method for production of natural compounds. This study isolated a strain of Rhizopus oryzae that could produce β-glucosidase, which efficiently hydrolyzes sophoricoside into genistein, from an enrichment culture of the dried fruits of Sophora japonica. After the composition of enzyme-producing medium and biotransformation conditions were optimized, a genistein yield of 85.6% was obtained after 24 h in a shake-flask biotransformation at pH 7.0 using an initial substrate concentration of 1 g/L. The developed process provides an alternative method for production of genistein, and would be suitable for scale-up production in the pharmaceutical industry.
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spelling pubmed-64840792019-05-13 A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza Mei, Jianfeng Chen, Xiang Liu, Jianghua Yi, Yu Zhang, Yanlu Ying, Guoqing Sci Rep Article Genistein is known to have multiple biological activities and has great potential for use as a preventative medicine and in disease treatment. Genistein can be extracted from plants, but also can be obtained from its glycoside form, sophoricoside, which is more abundant in some plants. Biotransformation by unpurified microbial enzymes has the advantage of low cost and is a preferred method for production of natural compounds. This study isolated a strain of Rhizopus oryzae that could produce β-glucosidase, which efficiently hydrolyzes sophoricoside into genistein, from an enrichment culture of the dried fruits of Sophora japonica. After the composition of enzyme-producing medium and biotransformation conditions were optimized, a genistein yield of 85.6% was obtained after 24 h in a shake-flask biotransformation at pH 7.0 using an initial substrate concentration of 1 g/L. The developed process provides an alternative method for production of genistein, and would be suitable for scale-up production in the pharmaceutical industry. Nature Publishing Group UK 2019-04-25 /pmc/articles/PMC6484079/ /pubmed/31024087 http://dx.doi.org/10.1038/s41598-019-42996-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Mei, Jianfeng
Chen, Xiang
Liu, Jianghua
Yi, Yu
Zhang, Yanlu
Ying, Guoqing
A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title_full A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title_fullStr A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title_full_unstemmed A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title_short A Biotransformation Process for Production of Genistein from Sophoricoside by a Strain of Rhizopus oryza
title_sort biotransformation process for production of genistein from sophoricoside by a strain of rhizopus oryza
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484079/
https://www.ncbi.nlm.nih.gov/pubmed/31024087
http://dx.doi.org/10.1038/s41598-019-42996-z
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