Top-down and middle-down proteomic analysis of Shiga toxin using MALDI-TOF-TOF mass spectrometry

The method describes a step-by-step process for analysis of putative Shiga toxin-producing Escherichia coli (STEC) for expression of Shiga toxin (Stx). The technique utilizes antibiotic induction, mass spectrometry and top-down/middle-down proteomic analysis. Stx expression is induced by overnight c...

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Detalles Bibliográficos
Autores principales: Fagerquist, Clifton K., Zaragoza, William J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484209/
https://www.ncbi.nlm.nih.gov/pubmed/31049298
http://dx.doi.org/10.1016/j.mex.2019.04.011

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