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miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect rem...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484313/ https://www.ncbi.nlm.nih.gov/pubmed/30761733 http://dx.doi.org/10.1111/jcmm.14220 |
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author | Sun, Zhongyang Li, Ying Wang, Han Cai, Min Gao, Shanshan Liu, Jing Tong, Liangcheng Hu, Zebing Wang, Yixuan Wang, Ke Zhang, Lijun Cao, Xinsheng Zhang, Shu Shi, Fei Zhao, Jianning |
author_facet | Sun, Zhongyang Li, Ying Wang, Han Cai, Min Gao, Shanshan Liu, Jing Tong, Liangcheng Hu, Zebing Wang, Yixuan Wang, Ke Zhang, Lijun Cao, Xinsheng Zhang, Shu Shi, Fei Zhao, Jianning |
author_sort | Sun, Zhongyang |
collection | PubMed |
description | Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect remains to be fully elucidated. Herein, we confirmed that simulated microgravity inhibits osteoblast proliferation. Then, we investigated the effect of mechanical unloading on the osteoblast cell cycle and found that simulated microgravity arrested the osteoblast cell cycle in the G(2) phase. In addition, our data showed that cell cycle arrest in osteoblasts from simulated microgravity was mainly because of decreased cyclin B1 expression. Furthermore, miR‐181c‐5p directly inhibited cyclin B1 protein translation by binding to a target site in the 3′UTR. Lastly, we demonstrated that inhibition of miR‐181c‐5p partially counteracted cell cycle arrest and decreased the osteoblast proliferation induced by simulated microgravity. In conclusion, our study demonstrates that simulated microgravity inhibits cell proliferation and induces cell cycle arrest in the G(2) phase in primary mouse osteoblasts partially through the miR‐181c‐5p/cyclin B1 pathway. This work may provide a novel mechanism of microgravity‐induced detrimental effects on osteoblasts and offer a new avenue to further investigate bone loss induced by mechanical unloading. |
format | Online Article Text |
id | pubmed-6484313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64843132019-05-03 miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase Sun, Zhongyang Li, Ying Wang, Han Cai, Min Gao, Shanshan Liu, Jing Tong, Liangcheng Hu, Zebing Wang, Yixuan Wang, Ke Zhang, Lijun Cao, Xinsheng Zhang, Shu Shi, Fei Zhao, Jianning J Cell Mol Med Original Articles Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect remains to be fully elucidated. Herein, we confirmed that simulated microgravity inhibits osteoblast proliferation. Then, we investigated the effect of mechanical unloading on the osteoblast cell cycle and found that simulated microgravity arrested the osteoblast cell cycle in the G(2) phase. In addition, our data showed that cell cycle arrest in osteoblasts from simulated microgravity was mainly because of decreased cyclin B1 expression. Furthermore, miR‐181c‐5p directly inhibited cyclin B1 protein translation by binding to a target site in the 3′UTR. Lastly, we demonstrated that inhibition of miR‐181c‐5p partially counteracted cell cycle arrest and decreased the osteoblast proliferation induced by simulated microgravity. In conclusion, our study demonstrates that simulated microgravity inhibits cell proliferation and induces cell cycle arrest in the G(2) phase in primary mouse osteoblasts partially through the miR‐181c‐5p/cyclin B1 pathway. This work may provide a novel mechanism of microgravity‐induced detrimental effects on osteoblasts and offer a new avenue to further investigate bone loss induced by mechanical unloading. John Wiley and Sons Inc. 2019-02-14 2019-05 /pmc/articles/PMC6484313/ /pubmed/30761733 http://dx.doi.org/10.1111/jcmm.14220 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Sun, Zhongyang Li, Ying Wang, Han Cai, Min Gao, Shanshan Liu, Jing Tong, Liangcheng Hu, Zebing Wang, Yixuan Wang, Ke Zhang, Lijun Cao, Xinsheng Zhang, Shu Shi, Fei Zhao, Jianning miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title | miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title_full | miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title_fullStr | miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title_full_unstemmed | miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title_short | miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase |
title_sort | mir‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the g(2) phase |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484313/ https://www.ncbi.nlm.nih.gov/pubmed/30761733 http://dx.doi.org/10.1111/jcmm.14220 |
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