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miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase

Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect rem...

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Autores principales: Sun, Zhongyang, Li, Ying, Wang, Han, Cai, Min, Gao, Shanshan, Liu, Jing, Tong, Liangcheng, Hu, Zebing, Wang, Yixuan, Wang, Ke, Zhang, Lijun, Cao, Xinsheng, Zhang, Shu, Shi, Fei, Zhao, Jianning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484313/
https://www.ncbi.nlm.nih.gov/pubmed/30761733
http://dx.doi.org/10.1111/jcmm.14220
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author Sun, Zhongyang
Li, Ying
Wang, Han
Cai, Min
Gao, Shanshan
Liu, Jing
Tong, Liangcheng
Hu, Zebing
Wang, Yixuan
Wang, Ke
Zhang, Lijun
Cao, Xinsheng
Zhang, Shu
Shi, Fei
Zhao, Jianning
author_facet Sun, Zhongyang
Li, Ying
Wang, Han
Cai, Min
Gao, Shanshan
Liu, Jing
Tong, Liangcheng
Hu, Zebing
Wang, Yixuan
Wang, Ke
Zhang, Lijun
Cao, Xinsheng
Zhang, Shu
Shi, Fei
Zhao, Jianning
author_sort Sun, Zhongyang
collection PubMed
description Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect remains to be fully elucidated. Herein, we confirmed that simulated microgravity inhibits osteoblast proliferation. Then, we investigated the effect of mechanical unloading on the osteoblast cell cycle and found that simulated microgravity arrested the osteoblast cell cycle in the G(2) phase. In addition, our data showed that cell cycle arrest in osteoblasts from simulated microgravity was mainly because of decreased cyclin B1 expression. Furthermore, miR‐181c‐5p directly inhibited cyclin B1 protein translation by binding to a target site in the 3′UTR. Lastly, we demonstrated that inhibition of miR‐181c‐5p partially counteracted cell cycle arrest and decreased the osteoblast proliferation induced by simulated microgravity. In conclusion, our study demonstrates that simulated microgravity inhibits cell proliferation and induces cell cycle arrest in the G(2) phase in primary mouse osteoblasts partially through the miR‐181c‐5p/cyclin B1 pathway. This work may provide a novel mechanism of microgravity‐induced detrimental effects on osteoblasts and offer a new avenue to further investigate bone loss induced by mechanical unloading.
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spelling pubmed-64843132019-05-03 miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase Sun, Zhongyang Li, Ying Wang, Han Cai, Min Gao, Shanshan Liu, Jing Tong, Liangcheng Hu, Zebing Wang, Yixuan Wang, Ke Zhang, Lijun Cao, Xinsheng Zhang, Shu Shi, Fei Zhao, Jianning J Cell Mol Med Original Articles Impaired osteoblast proliferation plays fundamental roles in microgravity‐induced bone loss, and cell cycle imbalance may result in abnormal osteoblast proliferation. However, whether microgravity exerts an influence on the cell cycle in osteoblasts or what mechanisms may underlie such an effect remains to be fully elucidated. Herein, we confirmed that simulated microgravity inhibits osteoblast proliferation. Then, we investigated the effect of mechanical unloading on the osteoblast cell cycle and found that simulated microgravity arrested the osteoblast cell cycle in the G(2) phase. In addition, our data showed that cell cycle arrest in osteoblasts from simulated microgravity was mainly because of decreased cyclin B1 expression. Furthermore, miR‐181c‐5p directly inhibited cyclin B1 protein translation by binding to a target site in the 3′UTR. Lastly, we demonstrated that inhibition of miR‐181c‐5p partially counteracted cell cycle arrest and decreased the osteoblast proliferation induced by simulated microgravity. In conclusion, our study demonstrates that simulated microgravity inhibits cell proliferation and induces cell cycle arrest in the G(2) phase in primary mouse osteoblasts partially through the miR‐181c‐5p/cyclin B1 pathway. This work may provide a novel mechanism of microgravity‐induced detrimental effects on osteoblasts and offer a new avenue to further investigate bone loss induced by mechanical unloading. John Wiley and Sons Inc. 2019-02-14 2019-05 /pmc/articles/PMC6484313/ /pubmed/30761733 http://dx.doi.org/10.1111/jcmm.14220 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Sun, Zhongyang
Li, Ying
Wang, Han
Cai, Min
Gao, Shanshan
Liu, Jing
Tong, Liangcheng
Hu, Zebing
Wang, Yixuan
Wang, Ke
Zhang, Lijun
Cao, Xinsheng
Zhang, Shu
Shi, Fei
Zhao, Jianning
miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title_full miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title_fullStr miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title_full_unstemmed miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title_short miR‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the G(2) phase
title_sort mir‐181c‐5p mediates simulated microgravity‐induced impaired osteoblast proliferation by promoting cell cycle arrested in the g(2) phase
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6484313/
https://www.ncbi.nlm.nih.gov/pubmed/30761733
http://dx.doi.org/10.1111/jcmm.14220
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