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Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity
BACKGROUND: Stevia rebaudiana Bertoni has various pharmacological actions, which includes antidiabetic, antioxidant, anti-inflammatory activities. The superoxide and consequently NADPH oxidase (Nox) are relevant targets involved in biological effects of Stevia. The presence of NADPH-containing super...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485107/ https://www.ncbi.nlm.nih.gov/pubmed/31023287 http://dx.doi.org/10.1186/s12906-019-2500-1 |
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author | Isoyan, A. S. Simonyan, K. V. Simonyan, R. M. Babayan, M. A. Simonyan, G. M. Chavushyan, V. A. Simonyan, M. A. |
author_facet | Isoyan, A. S. Simonyan, K. V. Simonyan, R. M. Babayan, M. A. Simonyan, G. M. Chavushyan, V. A. Simonyan, M. A. |
author_sort | Isoyan, A. S. |
collection | PubMed |
description | BACKGROUND: Stevia rebaudiana Bertoni has various pharmacological actions, which includes antidiabetic, antioxidant, anti-inflammatory activities. The superoxide and consequently NADPH oxidase (Nox) are relevant targets involved in biological effects of Stevia. The presence of NADPH-containing superoxide-producing lipoprotein (suprol) in Stevia leaves has not yet been tested. The mechanism of producing superoxide radicals (O(2)(−)) by suprol was determined in vitro, which is associated with the electron transfer from NADPH in the composition of suprol by traces of transition metal ions (Fe(3+) or Cu(2+)) to molecular oxygen, turning it into O(2)(−). It is expected that the therapeutic efficacy of Stevia leaves is caused by specific activity of superoxide-producing lipoprotein fraction. METHODS: For the first time, from the dry leaves of Stevia the NADPH-containing superoxide-producing lipoprotein was isolated and purified. The specific content of suprol (milligrams in 1 g of Stevia leaves- mg/g) was determined after desalination of suprol and lyophilization. RESULTS: According to the method provided, the specific content of the isolated suprol from Stevia’s leaves was up to 4.5 ± 0.2 mg / g (yields up to 68.5 ± 4.7%, p < 0.05, n = 6). Nox forms a stable complex with suprol. The optical absorption spectrum of the Nox-suprol complex represents the overlapping suprol and Nox spectra, with a certain background increase and characteristic features of optical absorption for Nox. Due to O(2)(−) producing capacity suprol-Nox complex discolors KMnO(4) solutions, Coomassie brilliant blue, restores nitrotetrazolium blue to formazan and oxidizes epinephrine to adrenochrome. The oxidation activity of adrenaline is 50.3 ± 5.1 U / mg / ml (p < 0.05, n = 6). CONCLUSION: Superoxide-producing lipoprotein fraction-Nox complex from Stevia leaves (membranes) can modulate redox regulated signaling pathways and may play a positive role in type-2 diabetes by means of adrenaline oxidation mechanism. |
format | Online Article Text |
id | pubmed-6485107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-64851072019-05-03 Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity Isoyan, A. S. Simonyan, K. V. Simonyan, R. M. Babayan, M. A. Simonyan, G. M. Chavushyan, V. A. Simonyan, M. A. BMC Complement Altern Med Research Article BACKGROUND: Stevia rebaudiana Bertoni has various pharmacological actions, which includes antidiabetic, antioxidant, anti-inflammatory activities. The superoxide and consequently NADPH oxidase (Nox) are relevant targets involved in biological effects of Stevia. The presence of NADPH-containing superoxide-producing lipoprotein (suprol) in Stevia leaves has not yet been tested. The mechanism of producing superoxide radicals (O(2)(−)) by suprol was determined in vitro, which is associated with the electron transfer from NADPH in the composition of suprol by traces of transition metal ions (Fe(3+) or Cu(2+)) to molecular oxygen, turning it into O(2)(−). It is expected that the therapeutic efficacy of Stevia leaves is caused by specific activity of superoxide-producing lipoprotein fraction. METHODS: For the first time, from the dry leaves of Stevia the NADPH-containing superoxide-producing lipoprotein was isolated and purified. The specific content of suprol (milligrams in 1 g of Stevia leaves- mg/g) was determined after desalination of suprol and lyophilization. RESULTS: According to the method provided, the specific content of the isolated suprol from Stevia’s leaves was up to 4.5 ± 0.2 mg / g (yields up to 68.5 ± 4.7%, p < 0.05, n = 6). Nox forms a stable complex with suprol. The optical absorption spectrum of the Nox-suprol complex represents the overlapping suprol and Nox spectra, with a certain background increase and characteristic features of optical absorption for Nox. Due to O(2)(−) producing capacity suprol-Nox complex discolors KMnO(4) solutions, Coomassie brilliant blue, restores nitrotetrazolium blue to formazan and oxidizes epinephrine to adrenochrome. The oxidation activity of adrenaline is 50.3 ± 5.1 U / mg / ml (p < 0.05, n = 6). CONCLUSION: Superoxide-producing lipoprotein fraction-Nox complex from Stevia leaves (membranes) can modulate redox regulated signaling pathways and may play a positive role in type-2 diabetes by means of adrenaline oxidation mechanism. BioMed Central 2019-04-25 /pmc/articles/PMC6485107/ /pubmed/31023287 http://dx.doi.org/10.1186/s12906-019-2500-1 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Isoyan, A. S. Simonyan, K. V. Simonyan, R. M. Babayan, M. A. Simonyan, G. M. Chavushyan, V. A. Simonyan, M. A. Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title | Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title_full | Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title_fullStr | Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title_full_unstemmed | Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title_short | Superoxide-producing lipoprotein fraction from Stevia leaves: definition of specific activity |
title_sort | superoxide-producing lipoprotein fraction from stevia leaves: definition of specific activity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485107/ https://www.ncbi.nlm.nih.gov/pubmed/31023287 http://dx.doi.org/10.1186/s12906-019-2500-1 |
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