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Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.

BACKGROUND: Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, cau...

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Autores principales: Kumarasingha, Rasika, Young, Neil D., Yeo, Tiong-Chia, Lim, Diana S. L., Tu, Chu-Lee, Palombo, Enzo A., Shaw, Jillian M., Gasser, Robin B., Boag, Peter R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485125/
https://www.ncbi.nlm.nih.gov/pubmed/31023350
http://dx.doi.org/10.1186/s13071-019-3429-4
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author Kumarasingha, Rasika
Young, Neil D.
Yeo, Tiong-Chia
Lim, Diana S. L.
Tu, Chu-Lee
Palombo, Enzo A.
Shaw, Jillian M.
Gasser, Robin B.
Boag, Peter R.
author_facet Kumarasingha, Rasika
Young, Neil D.
Yeo, Tiong-Chia
Lim, Diana S. L.
Tu, Chu-Lee
Palombo, Enzo A.
Shaw, Jillian M.
Gasser, Robin B.
Boag, Peter R.
author_sort Kumarasingha, Rasika
collection PubMed
description BACKGROUND: Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, causing quite pronounced stress responses in this nematode. We have also shown that a fraction, designated Pf-fraction 5, derived from this plant has a substantial adverse effect on this worm; however, nothing is known about the molecular processes affected in the worm. In the present study, we explored this aspect. RESULTS: Key biological processes linked to upregulated genes (n = 214) included ‘response to endoplasmic reticulum stress’ and ‘lipid metabolism’, and processes representing downregulated genes (n = 357) included ‘DNA-conformation change’ and ‘cellular lipid metabolism’. CONCLUSIONS: Exposure of C. elegans to Pf-fraction 5 induces significant changes in the transcriptome. Gene ontology analysis suggests that Pf-fraction 5 induces endoplasmic reticulum and mitochondrial stress, and the changes in gene expression are either a direct or indirect consequence of this. Further work is required to assess specific responses to sub-fractions of Pf-fraction 5 in time-course experiments in C. elegans, to define the chemical(s) with potent anthelmintic properties, to attempt to unravel their mode(s) of action and to assess their selectivity against nematodes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3429-4) contains supplementary material, which is available to authorized users.
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spelling pubmed-64851252019-05-03 Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour. Kumarasingha, Rasika Young, Neil D. Yeo, Tiong-Chia Lim, Diana S. L. Tu, Chu-Lee Palombo, Enzo A. Shaw, Jillian M. Gasser, Robin B. Boag, Peter R. Parasit Vectors Research BACKGROUND: Natural compounds from plants are known to provide a source of anthelmintic molecules. In previous studies, we have shown that plant extracts from the plant Picria fel-terrae Lour. and particular fractions thereof have activity against the free-living nematode Caenorhabditis elegans, causing quite pronounced stress responses in this nematode. We have also shown that a fraction, designated Pf-fraction 5, derived from this plant has a substantial adverse effect on this worm; however, nothing is known about the molecular processes affected in the worm. In the present study, we explored this aspect. RESULTS: Key biological processes linked to upregulated genes (n = 214) included ‘response to endoplasmic reticulum stress’ and ‘lipid metabolism’, and processes representing downregulated genes (n = 357) included ‘DNA-conformation change’ and ‘cellular lipid metabolism’. CONCLUSIONS: Exposure of C. elegans to Pf-fraction 5 induces significant changes in the transcriptome. Gene ontology analysis suggests that Pf-fraction 5 induces endoplasmic reticulum and mitochondrial stress, and the changes in gene expression are either a direct or indirect consequence of this. Further work is required to assess specific responses to sub-fractions of Pf-fraction 5 in time-course experiments in C. elegans, to define the chemical(s) with potent anthelmintic properties, to attempt to unravel their mode(s) of action and to assess their selectivity against nematodes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13071-019-3429-4) contains supplementary material, which is available to authorized users. BioMed Central 2019-04-25 /pmc/articles/PMC6485125/ /pubmed/31023350 http://dx.doi.org/10.1186/s13071-019-3429-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kumarasingha, Rasika
Young, Neil D.
Yeo, Tiong-Chia
Lim, Diana S. L.
Tu, Chu-Lee
Palombo, Enzo A.
Shaw, Jillian M.
Gasser, Robin B.
Boag, Peter R.
Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title_full Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title_fullStr Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title_full_unstemmed Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title_short Transcriptional alterations in Caenorhabditis elegans following exposure to an anthelmintic fraction of the plant Picria fel-terrae Lour.
title_sort transcriptional alterations in caenorhabditis elegans following exposure to an anthelmintic fraction of the plant picria fel-terrae lour.
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485125/
https://www.ncbi.nlm.nih.gov/pubmed/31023350
http://dx.doi.org/10.1186/s13071-019-3429-4
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