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Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis
BALB/c mice immunized with human cartilage proteoglycan (PG) develop arthritis accompanied by the production of autoantibodies to mouse cartilage PG. To determine whether the autoantibody isotype contributes to the onset and severity of arthritis, PG-specific serum IgG1 (Th2, IL-4-cytokine-supportin...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2002
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64852/ https://www.ncbi.nlm.nih.gov/pubmed/11879537 |
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author | Kaplan, Charles Valdez, Juan C Chandrasekaran, Raman Eibel, Hermann Mikecz, Katalin Glant, Tibor T Finnegan, Alison |
author_facet | Kaplan, Charles Valdez, Juan C Chandrasekaran, Raman Eibel, Hermann Mikecz, Katalin Glant, Tibor T Finnegan, Alison |
author_sort | Kaplan, Charles |
collection | PubMed |
description | BALB/c mice immunized with human cartilage proteoglycan (PG) develop arthritis accompanied by the production of autoantibodies to mouse cartilage PG. To determine whether the autoantibody isotype contributes to the onset and severity of arthritis, PG-specific serum IgG1 (Th2, IL-4-cytokine-supporting) and IgG2a (Th1, IFN-γ-controlling) concentrations were monitored during immunization with PG in IL-4-deficient and IFN-γ-deficient mice. Paradoxically, despite elevated IFN-γ, the PG-specific IgG1 isotype was significantly higher than the PG-specific IgG2a response, and the PG-specific IgG1 isotype was independent of IL-4. In contrast, the serum concentration of PG-specific IgG2a isotype was six times higher in IL-4-deficient mice than in wild-type controls. Moreover, the high concentration of PG-specific IgG2a isotype in IL-4-deficient mice corresponded to an increased severity of arthritis. The concentration of PG-specific IgG2a isotype was lower in IFN-γ-deficient mice than in wild-type mice, and the incidence and severity of arthritis also were significantly lower. Concentrations of PG-specific IgG2a isotype autoantibody correlated with the onset and severity of arthritis, suggesting a pathological role of this isotype, probably locally in the joint. |
format | Text |
id | pubmed-64852 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2002 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-648522002-01-25 Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis Kaplan, Charles Valdez, Juan C Chandrasekaran, Raman Eibel, Hermann Mikecz, Katalin Glant, Tibor T Finnegan, Alison Arthritis Res Research Article BALB/c mice immunized with human cartilage proteoglycan (PG) develop arthritis accompanied by the production of autoantibodies to mouse cartilage PG. To determine whether the autoantibody isotype contributes to the onset and severity of arthritis, PG-specific serum IgG1 (Th2, IL-4-cytokine-supporting) and IgG2a (Th1, IFN-γ-controlling) concentrations were monitored during immunization with PG in IL-4-deficient and IFN-γ-deficient mice. Paradoxically, despite elevated IFN-γ, the PG-specific IgG1 isotype was significantly higher than the PG-specific IgG2a response, and the PG-specific IgG1 isotype was independent of IL-4. In contrast, the serum concentration of PG-specific IgG2a isotype was six times higher in IL-4-deficient mice than in wild-type controls. Moreover, the high concentration of PG-specific IgG2a isotype in IL-4-deficient mice corresponded to an increased severity of arthritis. The concentration of PG-specific IgG2a isotype was lower in IFN-γ-deficient mice than in wild-type mice, and the incidence and severity of arthritis also were significantly lower. Concentrations of PG-specific IgG2a isotype autoantibody correlated with the onset and severity of arthritis, suggesting a pathological role of this isotype, probably locally in the joint. BioMed Central 2002 2001-10-02 /pmc/articles/PMC64852/ /pubmed/11879537 Text en Copyright © 2001 Kaplan et al, licensee BioMed Central Ltd |
spellingShingle | Research Article Kaplan, Charles Valdez, Juan C Chandrasekaran, Raman Eibel, Hermann Mikecz, Katalin Glant, Tibor T Finnegan, Alison Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title | Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title_full | Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title_fullStr | Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title_full_unstemmed | Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title_short | Th1 and Th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
title_sort | th1 and th2 cytokines regulate proteoglycan-specific autoantibody isotypes and arthritis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC64852/ https://www.ncbi.nlm.nih.gov/pubmed/11879537 |
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