Identification of optimal endogenous reference RNAs for RT-qPCR normalization in hindgut of rat models with anorectal malformations

BACKGROUND: Quantitative real-time polymerase chain reaction (RT-qPCR) is a sensitive method for quantifying mRNA abundance. With relative expression analysis, however, reliable data output is dependent on stably expressed reference genes across the samples being studied. In anorectal malformations...

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Detalles Bibliográficos
Autores principales: Long, Caiyun, Xiao, Yunxia, Li, Siying, Tang, Xiaobing, Yuan, Zhengwei, Bai, Yuzuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485207/
https://www.ncbi.nlm.nih.gov/pubmed/31065464
http://dx.doi.org/10.7717/peerj.6829

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