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Maternal circadian disruption is associated with variation in placental DNA methylation
Circadian disruption is a common environmental and occupational exposure with public health consequences, but not much is known about whether circadian disruption affects in utero development. We investigated whether maternal circadian disruption, using night shift work as a proxy, is associated wit...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485638/ https://www.ncbi.nlm.nih.gov/pubmed/31026301 http://dx.doi.org/10.1371/journal.pone.0215745 |
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author | Clarkson-Townsend, Danielle A. Everson, Todd M. Deyssenroth, Maya A. Burt, Amber A. Hermetz, Karen E. Hao, Ke Chen, Jia Marsit, Carmen J. |
author_facet | Clarkson-Townsend, Danielle A. Everson, Todd M. Deyssenroth, Maya A. Burt, Amber A. Hermetz, Karen E. Hao, Ke Chen, Jia Marsit, Carmen J. |
author_sort | Clarkson-Townsend, Danielle A. |
collection | PubMed |
description | Circadian disruption is a common environmental and occupational exposure with public health consequences, but not much is known about whether circadian disruption affects in utero development. We investigated whether maternal circadian disruption, using night shift work as a proxy, is associated with variations in DNA methylation patterns of placental tissue in an epigenome-wide association study (EWAS) of night shift work. Here, we compared cytosine-guanosine dinucleotide (CpG) specific methylation genome-wide of placental tissue (measured with the Illumina 450K array) from participants (n = 237) in the Rhode Island Child Health Study (RICHS) who did (n = 53) and did not (n = 184) report working the night shift, using robust linear modeling and adjusting for maternal age, pre-pregnancy smoking, infant sex, maternal adversity, and putative cell mixture. Statistical analyses were adjusted for multiple comparisons and results presented with Bonferroni or Benjamini and Hochberg (BH) adjustment for false discovery rate. Night shift work was associated with differential methylation in placental tissue, including CpG sites in the genes NAV1, SMPD1, TAPBP, CLEC16A, DIP2C, FAM172A, and PLEKHG6 (Bonferroni-adjusted p<0.05). CpG sites within NAV1, MXRA8, GABRG1, PRDM16, WNT5A, and FOXG1 exhibited the most hypomethylation, while CpG sites within TDO2, ADAMTSL3, DLX2, and SERPINA1 exhibited the most hypermethylation (BH q<0.10). Functional analysis indicated GO-terms associated with cell-cell adhesion and enriched GWAS results for psoriasis. Night shift work was associated with differential methylation of the placenta, which may have implications for fetal health and development. This is the first study to examine the epigenetic impacts of night shift exposure, as a proxy for circadian disruption, on placental methylation in humans, and, while results should be interpreted with caution, suggests circadian disruption may have epigenetic impacts. |
format | Online Article Text |
id | pubmed-6485638 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-64856382019-05-09 Maternal circadian disruption is associated with variation in placental DNA methylation Clarkson-Townsend, Danielle A. Everson, Todd M. Deyssenroth, Maya A. Burt, Amber A. Hermetz, Karen E. Hao, Ke Chen, Jia Marsit, Carmen J. PLoS One Research Article Circadian disruption is a common environmental and occupational exposure with public health consequences, but not much is known about whether circadian disruption affects in utero development. We investigated whether maternal circadian disruption, using night shift work as a proxy, is associated with variations in DNA methylation patterns of placental tissue in an epigenome-wide association study (EWAS) of night shift work. Here, we compared cytosine-guanosine dinucleotide (CpG) specific methylation genome-wide of placental tissue (measured with the Illumina 450K array) from participants (n = 237) in the Rhode Island Child Health Study (RICHS) who did (n = 53) and did not (n = 184) report working the night shift, using robust linear modeling and adjusting for maternal age, pre-pregnancy smoking, infant sex, maternal adversity, and putative cell mixture. Statistical analyses were adjusted for multiple comparisons and results presented with Bonferroni or Benjamini and Hochberg (BH) adjustment for false discovery rate. Night shift work was associated with differential methylation in placental tissue, including CpG sites in the genes NAV1, SMPD1, TAPBP, CLEC16A, DIP2C, FAM172A, and PLEKHG6 (Bonferroni-adjusted p<0.05). CpG sites within NAV1, MXRA8, GABRG1, PRDM16, WNT5A, and FOXG1 exhibited the most hypomethylation, while CpG sites within TDO2, ADAMTSL3, DLX2, and SERPINA1 exhibited the most hypermethylation (BH q<0.10). Functional analysis indicated GO-terms associated with cell-cell adhesion and enriched GWAS results for psoriasis. Night shift work was associated with differential methylation of the placenta, which may have implications for fetal health and development. This is the first study to examine the epigenetic impacts of night shift exposure, as a proxy for circadian disruption, on placental methylation in humans, and, while results should be interpreted with caution, suggests circadian disruption may have epigenetic impacts. Public Library of Science 2019-04-26 /pmc/articles/PMC6485638/ /pubmed/31026301 http://dx.doi.org/10.1371/journal.pone.0215745 Text en © 2019 Clarkson-Townsend et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Clarkson-Townsend, Danielle A. Everson, Todd M. Deyssenroth, Maya A. Burt, Amber A. Hermetz, Karen E. Hao, Ke Chen, Jia Marsit, Carmen J. Maternal circadian disruption is associated with variation in placental DNA methylation |
title | Maternal circadian disruption is associated with variation in placental DNA methylation |
title_full | Maternal circadian disruption is associated with variation in placental DNA methylation |
title_fullStr | Maternal circadian disruption is associated with variation in placental DNA methylation |
title_full_unstemmed | Maternal circadian disruption is associated with variation in placental DNA methylation |
title_short | Maternal circadian disruption is associated with variation in placental DNA methylation |
title_sort | maternal circadian disruption is associated with variation in placental dna methylation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6485638/ https://www.ncbi.nlm.nih.gov/pubmed/31026301 http://dx.doi.org/10.1371/journal.pone.0215745 |
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