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Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent

Medicinal plants have been considered as promising sources of drugs in treating various cancers. Crinum amabile (C. amabile), a plant species from the Amaryllidaceae family, is claimed to be a potential source for cancer chemotherapeutic compounds. Here, we aimed to investigate the potential of C. a...

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Autores principales: Lim, Chung Pin, Yam, Mun Fei, Asmawi, Mohd. Zaini, Chin, Voon Kin, Khairuddin, Nurul Hayah, Yong, Yoke Keong, Hassan, Haniza, Basir, Rusliza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487101/
https://www.ncbi.nlm.nih.gov/pubmed/31097973
http://dx.doi.org/10.1155/2019/7521504
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author Lim, Chung Pin
Yam, Mun Fei
Asmawi, Mohd. Zaini
Chin, Voon Kin
Khairuddin, Nurul Hayah
Yong, Yoke Keong
Hassan, Haniza
Basir, Rusliza
author_facet Lim, Chung Pin
Yam, Mun Fei
Asmawi, Mohd. Zaini
Chin, Voon Kin
Khairuddin, Nurul Hayah
Yong, Yoke Keong
Hassan, Haniza
Basir, Rusliza
author_sort Lim, Chung Pin
collection PubMed
description Medicinal plants have been considered as promising sources of drugs in treating various cancers. Crinum amabile (C. amabile), a plant species from the Amaryllidaceae family, is claimed to be a potential source for cancer chemotherapeutic compounds. Here, we aimed to investigate the potential of C. amabile as an anticancer agent. Dried leaves of C. amabile were serially extracted and our findings showed that chloroform extract (CE) was shown to exhibit cytotoxic effect against all cancer cell lines used. This active extract was further fractionated in which F5 fraction was shown to possess the highest cytotoxicity among all fractions. F5 fraction was then tested in-depth through Annexin V/FITC apoptosis and DNA fragmentation assays to determine its apoptotic effect on MCF-7 cells. Results revealed that F5 fraction only showed induction of cell apoptosis starting at 72-hour treatment while DNA fragmentation was not detected at any of the concentrations and treatment periods tested. Meanwhile, cell proliferation assay revealed that F5 fraction was able to inhibit normal cell proliferation as well as VEGF-induced cell proliferation of normal endothelial cell (HUVECs). In conclusion, F5 fraction from C. amabile leaf CE was able to exhibit cytostatic effect through antiproliferation activity rather than induction of cell apoptosis and therefore has the potential to be further investigated as an anticancer agent.
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spelling pubmed-64871012019-05-16 Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent Lim, Chung Pin Yam, Mun Fei Asmawi, Mohd. Zaini Chin, Voon Kin Khairuddin, Nurul Hayah Yong, Yoke Keong Hassan, Haniza Basir, Rusliza Evid Based Complement Alternat Med Research Article Medicinal plants have been considered as promising sources of drugs in treating various cancers. Crinum amabile (C. amabile), a plant species from the Amaryllidaceae family, is claimed to be a potential source for cancer chemotherapeutic compounds. Here, we aimed to investigate the potential of C. amabile as an anticancer agent. Dried leaves of C. amabile were serially extracted and our findings showed that chloroform extract (CE) was shown to exhibit cytotoxic effect against all cancer cell lines used. This active extract was further fractionated in which F5 fraction was shown to possess the highest cytotoxicity among all fractions. F5 fraction was then tested in-depth through Annexin V/FITC apoptosis and DNA fragmentation assays to determine its apoptotic effect on MCF-7 cells. Results revealed that F5 fraction only showed induction of cell apoptosis starting at 72-hour treatment while DNA fragmentation was not detected at any of the concentrations and treatment periods tested. Meanwhile, cell proliferation assay revealed that F5 fraction was able to inhibit normal cell proliferation as well as VEGF-induced cell proliferation of normal endothelial cell (HUVECs). In conclusion, F5 fraction from C. amabile leaf CE was able to exhibit cytostatic effect through antiproliferation activity rather than induction of cell apoptosis and therefore has the potential to be further investigated as an anticancer agent. Hindawi 2019-04-11 /pmc/articles/PMC6487101/ /pubmed/31097973 http://dx.doi.org/10.1155/2019/7521504 Text en Copyright © 2019 Chung Pin Lim et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lim, Chung Pin
Yam, Mun Fei
Asmawi, Mohd. Zaini
Chin, Voon Kin
Khairuddin, Nurul Hayah
Yong, Yoke Keong
Hassan, Haniza
Basir, Rusliza
Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title_full Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title_fullStr Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title_full_unstemmed Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title_short Cytostatic and Antiproliferative Activities of F5 Fraction of Crinum amabile Leaf Chloroform Extract Showed Its Potential as Cancer Chemotherapeutic Agent
title_sort cytostatic and antiproliferative activities of f5 fraction of crinum amabile leaf chloroform extract showed its potential as cancer chemotherapeutic agent
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487101/
https://www.ncbi.nlm.nih.gov/pubmed/31097973
http://dx.doi.org/10.1155/2019/7521504
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