Cargando…

Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats

BACKGROUND AND AIMS: Hydrotalcite plays an important role in the therapy of gastric ulcer induced by nonsteroidal anti-inflammatory drugs (NSAIDs), but little is known about the mechanism. We designed two experiments to study the preventive and curative effects of hydrotalcite on NSAIDs-related gast...

Descripción completa

Detalles Bibliográficos
Autores principales: Fang, Yan Fei, Xu, Wen Li, Wang, Lan, Lian, Qing Wu, Qiu, Li Feng, Zhou, Hui, Chen, Shu Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487128/
https://www.ncbi.nlm.nih.gov/pubmed/31111054
http://dx.doi.org/10.1155/2019/4605748
_version_ 1783414447637790720
author Fang, Yan Fei
Xu, Wen Li
Wang, Lan
Lian, Qing Wu
Qiu, Li Feng
Zhou, Hui
Chen, Shu Jie
author_facet Fang, Yan Fei
Xu, Wen Li
Wang, Lan
Lian, Qing Wu
Qiu, Li Feng
Zhou, Hui
Chen, Shu Jie
author_sort Fang, Yan Fei
collection PubMed
description BACKGROUND AND AIMS: Hydrotalcite plays an important role in the therapy of gastric ulcer induced by nonsteroidal anti-inflammatory drugs (NSAIDs), but little is known about the mechanism. We designed two experiments to study the preventive and curative effects of hydrotalcite on NSAIDs-related gastric injury in rats and to investigate the relationship between the protective and curative mechanism of hydrotalcite and the secretion of epidermal growth factor (EGF)/prostaglandin E2 (PGE2). METHODS: Two experiments were separately designed to evaluate the preventive and curative effects of hydrotalcite. A total of 25 male rats and 25 female rats were randomly divided into five groups (vehicle group, model group, omeprazole group, hydrotalcite group, and ranitidine group) in each experiment. Rats were treated with indomethacin by gavage to build the model of acute gastric mucosal injury. The concentrations of EGF and PGE2 in blood specimens and mucosal injury indexes by gross inspection were measured and an immunohistochemical technique was also employed to test the levels of EGF, cyclooxygenase-1 (COX-1), and cyclooxygenase-2 (COX-2) in gastric mucosa. RESULTS: Comparing with model group in both preventive and curative experiments, hydrotalcite decreased the gastric injury in the mucosa of stomach significantly (7±4.5 vs. 16±11.25, 1.5±2 vs. 2.5±6; P<0.01, P<0.05). The levels of EGF and PGE2 in blood serum were markedly higher in hydrotalcite group than that in model group and ranitidine group in preventive experiment (574.39±34.28 vs. 486.22±41.73, 488.07±24.44; P<0.01, P<0.01). The expression levels of COX-2 in gastric mucosa were also higher in hydrotalcite group than that in model group in both preventive and therapeutic experiments (12±4 vs. 9±6, 14±7 vs. 9±4; P<0.01, P<0.05). CONCLUSIONS: Hydrotalcite promotes gastric protection and healing via several mechanisms, including increased levels of PGE2 in blood serum, activation of EGF, and antagonising the inhibition of cyclooxygenase (COX) caused by NSAIDs.
format Online
Article
Text
id pubmed-6487128
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-64871282019-05-20 Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats Fang, Yan Fei Xu, Wen Li Wang, Lan Lian, Qing Wu Qiu, Li Feng Zhou, Hui Chen, Shu Jie Biomed Res Int Research Article BACKGROUND AND AIMS: Hydrotalcite plays an important role in the therapy of gastric ulcer induced by nonsteroidal anti-inflammatory drugs (NSAIDs), but little is known about the mechanism. We designed two experiments to study the preventive and curative effects of hydrotalcite on NSAIDs-related gastric injury in rats and to investigate the relationship between the protective and curative mechanism of hydrotalcite and the secretion of epidermal growth factor (EGF)/prostaglandin E2 (PGE2). METHODS: Two experiments were separately designed to evaluate the preventive and curative effects of hydrotalcite. A total of 25 male rats and 25 female rats were randomly divided into five groups (vehicle group, model group, omeprazole group, hydrotalcite group, and ranitidine group) in each experiment. Rats were treated with indomethacin by gavage to build the model of acute gastric mucosal injury. The concentrations of EGF and PGE2 in blood specimens and mucosal injury indexes by gross inspection were measured and an immunohistochemical technique was also employed to test the levels of EGF, cyclooxygenase-1 (COX-1), and cyclooxygenase-2 (COX-2) in gastric mucosa. RESULTS: Comparing with model group in both preventive and curative experiments, hydrotalcite decreased the gastric injury in the mucosa of stomach significantly (7±4.5 vs. 16±11.25, 1.5±2 vs. 2.5±6; P<0.01, P<0.05). The levels of EGF and PGE2 in blood serum were markedly higher in hydrotalcite group than that in model group and ranitidine group in preventive experiment (574.39±34.28 vs. 486.22±41.73, 488.07±24.44; P<0.01, P<0.01). The expression levels of COX-2 in gastric mucosa were also higher in hydrotalcite group than that in model group in both preventive and therapeutic experiments (12±4 vs. 9±6, 14±7 vs. 9±4; P<0.01, P<0.05). CONCLUSIONS: Hydrotalcite promotes gastric protection and healing via several mechanisms, including increased levels of PGE2 in blood serum, activation of EGF, and antagonising the inhibition of cyclooxygenase (COX) caused by NSAIDs. Hindawi 2019-04-11 /pmc/articles/PMC6487128/ /pubmed/31111054 http://dx.doi.org/10.1155/2019/4605748 Text en Copyright © 2019 Yan Fei Fang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Fang, Yan Fei
Xu, Wen Li
Wang, Lan
Lian, Qing Wu
Qiu, Li Feng
Zhou, Hui
Chen, Shu Jie
Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title_full Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title_fullStr Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title_full_unstemmed Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title_short Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats
title_sort effect of hydrotalcite on indometacin-induced gastric injury in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487128/
https://www.ncbi.nlm.nih.gov/pubmed/31111054
http://dx.doi.org/10.1155/2019/4605748
work_keys_str_mv AT fangyanfei effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT xuwenli effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT wanglan effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT lianqingwu effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT qiulifeng effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT zhouhui effectofhydrotalciteonindometacininducedgastricinjuryinrats
AT chenshujie effectofhydrotalciteonindometacininducedgastricinjuryinrats