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Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016
AIM: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. MATERIALS AND METHODS: We collected 300 tissue specimens, including the trachea, bronchi,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487242/ https://www.ncbi.nlm.nih.gov/pubmed/31089313 http://dx.doi.org/10.14202/vetworld.2019.424-433 |
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author | Alsultan, Musaed Abdulaziz Alhammadi, Mohamed Ali Hemida, Maged Gomaa |
author_facet | Alsultan, Musaed Abdulaziz Alhammadi, Mohamed Ali Hemida, Maged Gomaa |
author_sort | Alsultan, Musaed Abdulaziz |
collection | PubMed |
description | AIM: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. MATERIALS AND METHODS: We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences. RESULTS: Our molecular surveillance revealed that 31.6% of the tested specimens were IBV positive by PCR. We selected some positive specimens showing low Ct values by the qRT-PCR for virus isolation by the ECE. The infected eggs showed hemorrhage, dwarfing, and death in some cases after three passages in the ECE. We sequenced some of the positive PCR specimens and used the obtained sequences to draw the phylogenetic tree based on the partial IBV-ORF-1a, N, and S1 gene sequences. The phylogenetic trees based on the IBV-N and S1 gene sequences showed that the circulating IBV strains in Al-Hasa during 2016 was showing a high degree of identity to some strains from Taiwan and Italy. Meanwhile, the grouping of these strains based on the IBV-S1 sequences revealed that the currently circulating IBV strains in Al-Hasa belonged to Gr.I.7 along with strains from Taiwan. CONCLUSION: Our results confirmed the continuous circulation of the IBV among the chicken population in Al-Hasa despite the intensive application of vaccines against this virus. |
format | Online Article Text |
id | pubmed-6487242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-64872422019-05-14 Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 Alsultan, Musaed Abdulaziz Alhammadi, Mohamed Ali Hemida, Maged Gomaa Vet World Research Article AIM: This study aimed to isolate some of the currently circulating infectious bronchitis virus (IBV) strains from some broiler chicken farms in Al-Hasa and to do some molecular characteristics of these strains. MATERIALS AND METHODS: We collected 300 tissue specimens, including the trachea, bronchi, lungs, and kidneys from some four commercial chicken farms showing respiratory manifestations. We tested these tissue specimens by the real-time polymerase chain reaction (RT-PCR) and gel-based PCR. We selected some PCR positive samples for isolation in the embryonated chicken eggs (ECE). We sequenced some PCR-positive samples and conducted phylogenetic analysis based on the obtained sequences. RESULTS: Our molecular surveillance revealed that 31.6% of the tested specimens were IBV positive by PCR. We selected some positive specimens showing low Ct values by the qRT-PCR for virus isolation by the ECE. The infected eggs showed hemorrhage, dwarfing, and death in some cases after three passages in the ECE. We sequenced some of the positive PCR specimens and used the obtained sequences to draw the phylogenetic tree based on the partial IBV-ORF-1a, N, and S1 gene sequences. The phylogenetic trees based on the IBV-N and S1 gene sequences showed that the circulating IBV strains in Al-Hasa during 2016 was showing a high degree of identity to some strains from Taiwan and Italy. Meanwhile, the grouping of these strains based on the IBV-S1 sequences revealed that the currently circulating IBV strains in Al-Hasa belonged to Gr.I.7 along with strains from Taiwan. CONCLUSION: Our results confirmed the continuous circulation of the IBV among the chicken population in Al-Hasa despite the intensive application of vaccines against this virus. Veterinary World 2019 2019-03-19 /pmc/articles/PMC6487242/ /pubmed/31089313 http://dx.doi.org/10.14202/vetworld.2019.424-433 Text en Copyright: © Alsultan, et al. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Alsultan, Musaed Abdulaziz Alhammadi, Mohamed Ali Hemida, Maged Gomaa Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title | Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title_full | Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title_fullStr | Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title_full_unstemmed | Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title_short | Infectious bronchitis virus from chickens in Al-Hasa, Saudi Arabia 2015-2016 |
title_sort | infectious bronchitis virus from chickens in al-hasa, saudi arabia 2015-2016 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487242/ https://www.ncbi.nlm.nih.gov/pubmed/31089313 http://dx.doi.org/10.14202/vetworld.2019.424-433 |
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