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Expression Patterns of Growth Related Genes in Juvenile Red Spotted Grouper (Epinephelus akaara) with Different Growth Performance after Size Grading
Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Society of Developmental Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487320/ https://www.ncbi.nlm.nih.gov/pubmed/31049470 http://dx.doi.org/10.12717/DR.2019.23.1.035 |
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author | Mun, Seong Hee You, Jin Ho Oh, Hyeon Ji Lee, Chi Hoon Baek, Hea Ja Lee, Young-Don Kwon, Joon Yeong |
author_facet | Mun, Seong Hee You, Jin Ho Oh, Hyeon Ji Lee, Chi Hoon Baek, Hea Ja Lee, Young-Don Kwon, Joon Yeong |
author_sort | Mun, Seong Hee |
collection | PubMed |
description | Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper (Epinephelus akaara) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature 24±1℃, natural light) for 96 days after hatching. They were divided into 3 groups by size (fast growing, middle growing and slow growing groups: FGG, MGG, and SGG, respectively). RNA was extracted from the brain, liver and muscle tissues from each group, and target gene expression was examined by real-time PCR. In the brain with pituitary gland, expression of GH gene in FGG was significantly higher than the expression in SGG, but the expression of IGF1 and GHR genes in the muscle was highest in SGG. Difference of GHR and IGF1 mRNA in the liver between groups with different growth performance was less clear than that in other tissues, although level of IGF1 mRNA was higher in SGG than in MGG. These results suggest that hormonal governing of growth is not the same in fast growing and slow growing fish, and size grading could cause a shift of hormonal state and growth pattern in this species. |
format | Online Article Text |
id | pubmed-6487320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Korean Society of Developmental Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-64873202019-05-02 Expression Patterns of Growth Related Genes in Juvenile Red Spotted Grouper (Epinephelus akaara) with Different Growth Performance after Size Grading Mun, Seong Hee You, Jin Ho Oh, Hyeon Ji Lee, Chi Hoon Baek, Hea Ja Lee, Young-Don Kwon, Joon Yeong Dev Reprod Original Research Paper Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper (Epinephelus akaara) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature 24±1℃, natural light) for 96 days after hatching. They were divided into 3 groups by size (fast growing, middle growing and slow growing groups: FGG, MGG, and SGG, respectively). RNA was extracted from the brain, liver and muscle tissues from each group, and target gene expression was examined by real-time PCR. In the brain with pituitary gland, expression of GH gene in FGG was significantly higher than the expression in SGG, but the expression of IGF1 and GHR genes in the muscle was highest in SGG. Difference of GHR and IGF1 mRNA in the liver between groups with different growth performance was less clear than that in other tissues, although level of IGF1 mRNA was higher in SGG than in MGG. These results suggest that hormonal governing of growth is not the same in fast growing and slow growing fish, and size grading could cause a shift of hormonal state and growth pattern in this species. Korean Society of Developmental Biology 2019-03 2019-03-31 /pmc/articles/PMC6487320/ /pubmed/31049470 http://dx.doi.org/10.12717/DR.2019.23.1.035 Text en © Copyright 2019 The Korean Society of Developmental Biology http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Paper Mun, Seong Hee You, Jin Ho Oh, Hyeon Ji Lee, Chi Hoon Baek, Hea Ja Lee, Young-Don Kwon, Joon Yeong Expression Patterns of Growth Related Genes in Juvenile Red Spotted Grouper (Epinephelus akaara) with Different Growth Performance after Size Grading |
title | Expression Patterns of Growth Related Genes in Juvenile Red Spotted
Grouper (Epinephelus akaara) with Different Growth Performance
after Size Grading |
title_full | Expression Patterns of Growth Related Genes in Juvenile Red Spotted
Grouper (Epinephelus akaara) with Different Growth Performance
after Size Grading |
title_fullStr | Expression Patterns of Growth Related Genes in Juvenile Red Spotted
Grouper (Epinephelus akaara) with Different Growth Performance
after Size Grading |
title_full_unstemmed | Expression Patterns of Growth Related Genes in Juvenile Red Spotted
Grouper (Epinephelus akaara) with Different Growth Performance
after Size Grading |
title_short | Expression Patterns of Growth Related Genes in Juvenile Red Spotted
Grouper (Epinephelus akaara) with Different Growth Performance
after Size Grading |
title_sort | expression patterns of growth related genes in juvenile red spotted
grouper (epinephelus akaara) with different growth performance
after size grading |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487320/ https://www.ncbi.nlm.nih.gov/pubmed/31049470 http://dx.doi.org/10.12717/DR.2019.23.1.035 |
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