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SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation

In this work, we investigated the loading and conjugation of Curcumin on oleic acid (OA) and citric acid (CA) functionalized iron oxide nanoparticles and its applications in improving contrast in MRI. Magnetic iron oxide nanoparticles (Fe(3)O(4), MNPs) were synthesized using the co-precipitation met...

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Autores principales: Rahimnia, Rasoul, Salehi, Zeinab, Shafiee Ardestani, Mehdi, Doosthoseini, Hamid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487395/
https://www.ncbi.nlm.nih.gov/pubmed/31089354
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author Rahimnia, Rasoul
Salehi, Zeinab
Shafiee Ardestani, Mehdi
Doosthoseini, Hamid
author_facet Rahimnia, Rasoul
Salehi, Zeinab
Shafiee Ardestani, Mehdi
Doosthoseini, Hamid
author_sort Rahimnia, Rasoul
collection PubMed
description In this work, we investigated the loading and conjugation of Curcumin on oleic acid (OA) and citric acid (CA) functionalized iron oxide nanoparticles and its applications in improving contrast in MRI. Magnetic iron oxide nanoparticles (Fe(3)O(4), MNPs) were synthesized using the co-precipitation method and characterized by XRD, DLS, FT-IR, VSM, and SEM. FT-IR results confirmed functionalization with oleic acid and citric acid. Curcumin was loaded and conjugated with the Nano-Systems and the amount of Curcumin loaded was quantified using spectrophotometry at 419 nm wavelength. The impact of solvent on the loading of Curcumin was studied. The wt% of loaded Curcumin was found to be 0.189 wt% using dimethylformamide (DMF) whereas using a combination of water-ethanol (15% v/v), this increased to 56.149 wt%. T2 relaxation time was determined using a 1.5 Tesla MRI machine; results showed that the MNPs reduced T2. Cytotoxicity of Nano-Systems (NS) in MTT assay showed that concentrations higher than 80 μg/mL (C(NS) > 80 μg/mL) could lead to cancer cell death and low concentrations, up to 40 μg/mL (C(NS) < 40 μg/mL) could be evaluated for diagnostic purposes.
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spelling pubmed-64873952019-05-14 SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation Rahimnia, Rasoul Salehi, Zeinab Shafiee Ardestani, Mehdi Doosthoseini, Hamid Iran J Pharm Res Original Article In this work, we investigated the loading and conjugation of Curcumin on oleic acid (OA) and citric acid (CA) functionalized iron oxide nanoparticles and its applications in improving contrast in MRI. Magnetic iron oxide nanoparticles (Fe(3)O(4), MNPs) were synthesized using the co-precipitation method and characterized by XRD, DLS, FT-IR, VSM, and SEM. FT-IR results confirmed functionalization with oleic acid and citric acid. Curcumin was loaded and conjugated with the Nano-Systems and the amount of Curcumin loaded was quantified using spectrophotometry at 419 nm wavelength. The impact of solvent on the loading of Curcumin was studied. The wt% of loaded Curcumin was found to be 0.189 wt% using dimethylformamide (DMF) whereas using a combination of water-ethanol (15% v/v), this increased to 56.149 wt%. T2 relaxation time was determined using a 1.5 Tesla MRI machine; results showed that the MNPs reduced T2. Cytotoxicity of Nano-Systems (NS) in MTT assay showed that concentrations higher than 80 μg/mL (C(NS) > 80 μg/mL) could lead to cancer cell death and low concentrations, up to 40 μg/mL (C(NS) < 40 μg/mL) could be evaluated for diagnostic purposes. Shaheed Beheshti University of Medical Sciences 2019 /pmc/articles/PMC6487395/ /pubmed/31089354 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rahimnia, Rasoul
Salehi, Zeinab
Shafiee Ardestani, Mehdi
Doosthoseini, Hamid
SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title_full SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title_fullStr SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title_full_unstemmed SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title_short SPION Conjugated Curcumin Nano-Imaging Probe: Synthesis and Bio-Physical Evaluation
title_sort spion conjugated curcumin nano-imaging probe: synthesis and bio-physical evaluation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6487395/
https://www.ncbi.nlm.nih.gov/pubmed/31089354
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