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EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL EVOLUTION
BACKGROUND: Metastasis is common in the diagnosis of pancreatic cancer, and the presence of epithelial-mesenchymal transition markers in circulating tumor cells may suggest worse prognosis. AIM: To correlate the number of circulating tumor cells (CTCs) in the peripheral blood of patients with a loca...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Cirurgia Digestiva
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488272/ https://www.ncbi.nlm.nih.gov/pubmed/31038558 http://dx.doi.org/10.1590/0102-672020190001e1433 |
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author | GASPARINI-JUNIOR, José Luiz FANELLI, Marcello Ferretti ABDALLAH, Emne Ali CHINEN, Ludmilla Thomé Domingos |
author_facet | GASPARINI-JUNIOR, José Luiz FANELLI, Marcello Ferretti ABDALLAH, Emne Ali CHINEN, Ludmilla Thomé Domingos |
author_sort | GASPARINI-JUNIOR, José Luiz |
collection | PubMed |
description | BACKGROUND: Metastasis is common in the diagnosis of pancreatic cancer, and the presence of epithelial-mesenchymal transition markers in circulating tumor cells may suggest worse prognosis. AIM: To correlate the number of circulating tumor cells (CTCs) in the peripheral blood of patients with a locally advanced or metastatic pancreatic tumor and the protein expression involved in epithelial-mesenchymal transition (EMT) in CTCs with clinical characteristics, progression-free survival (PFS) and overall survival (OS). METHOD: This was a prospective study conducted using peripheral blood samples collected at three different times. CTCs were quantified by the ISET test and analyzed by immunocytochemistry. Proteins involved in EMT (vimentin, TGFß-RI and MMP2) were analyzed in all CTCs. RESULTS: Twenty-one patients were included. Median CTCs detected were 22, 20 and 8 CTCs/8 ml blood at baseline, first and second follow-up, respectively. No statistically significant correlation was found in correlating the number of CTCs and the evaluated clinical characteristics, PFS, or OS. There was no difference in PFS and OS among the EMT markers in the groups with and without markers. CONCLUSION: CTC analysis was not relevant in this sample for comparing clinical findings, PFS and OS in patients with pancreatic cancer. However, marker analysis in CTCs could be useful for the MMP-2 and/or TGFß-RI expression, as observed by the separate PFS curve. |
format | Online Article Text |
id | pubmed-6488272 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Colégio Brasileiro de Cirurgia Digestiva |
record_format | MEDLINE/PubMed |
spelling | pubmed-64882722019-05-06 EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL EVOLUTION GASPARINI-JUNIOR, José Luiz FANELLI, Marcello Ferretti ABDALLAH, Emne Ali CHINEN, Ludmilla Thomé Domingos Arq Bras Cir Dig Original Article BACKGROUND: Metastasis is common in the diagnosis of pancreatic cancer, and the presence of epithelial-mesenchymal transition markers in circulating tumor cells may suggest worse prognosis. AIM: To correlate the number of circulating tumor cells (CTCs) in the peripheral blood of patients with a locally advanced or metastatic pancreatic tumor and the protein expression involved in epithelial-mesenchymal transition (EMT) in CTCs with clinical characteristics, progression-free survival (PFS) and overall survival (OS). METHOD: This was a prospective study conducted using peripheral blood samples collected at three different times. CTCs were quantified by the ISET test and analyzed by immunocytochemistry. Proteins involved in EMT (vimentin, TGFß-RI and MMP2) were analyzed in all CTCs. RESULTS: Twenty-one patients were included. Median CTCs detected were 22, 20 and 8 CTCs/8 ml blood at baseline, first and second follow-up, respectively. No statistically significant correlation was found in correlating the number of CTCs and the evaluated clinical characteristics, PFS, or OS. There was no difference in PFS and OS among the EMT markers in the groups with and without markers. CONCLUSION: CTC analysis was not relevant in this sample for comparing clinical findings, PFS and OS in patients with pancreatic cancer. However, marker analysis in CTCs could be useful for the MMP-2 and/or TGFß-RI expression, as observed by the separate PFS curve. Colégio Brasileiro de Cirurgia Digestiva 2019-04-29 /pmc/articles/PMC6488272/ /pubmed/31038558 http://dx.doi.org/10.1590/0102-672020190001e1433 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Original Article GASPARINI-JUNIOR, José Luiz FANELLI, Marcello Ferretti ABDALLAH, Emne Ali CHINEN, Ludmilla Thomé Domingos EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL EVOLUTION |
title | EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF
PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL
EVOLUTION |
title_full | EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF
PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL
EVOLUTION |
title_fullStr | EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF
PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL
EVOLUTION |
title_full_unstemmed | EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF
PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL
EVOLUTION |
title_short | EVALUATING MMP-2 AND TGFß-RI EXPRESSION IN CIRCULATING TUMOR CELLS OF
PANCREATIC CANCER PATIENTS AND THEIR CORRELATION WITH CLINICAL
EVOLUTION |
title_sort | evaluating mmp-2 and tgfß-ri expression in circulating tumor cells of
pancreatic cancer patients and their correlation with clinical
evolution |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488272/ https://www.ncbi.nlm.nih.gov/pubmed/31038558 http://dx.doi.org/10.1590/0102-672020190001e1433 |
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