Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis

Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many sp...

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Autores principales: Furusawa, Yoshikazu, Takei, Junko, Sayama, Yusuke, Yamada, Shinji, Kaneko, Mika K., Kato, Yukinari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488525/
https://www.ncbi.nlm.nih.gov/pubmed/31061899
http://dx.doi.org/10.1016/j.bbrep.2019.100644
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author Furusawa, Yoshikazu
Takei, Junko
Sayama, Yusuke
Yamada, Shinji
Kaneko, Mika K.
Kato, Yukinari
author_facet Furusawa, Yoshikazu
Takei, Junko
Sayama, Yusuke
Yamada, Shinji
Kaneko, Mika K.
Kato, Yukinari
author_sort Furusawa, Yoshikazu
collection PubMed
description Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN) has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN) cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG(1), kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN.
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spelling pubmed-64885252019-05-06 Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis Furusawa, Yoshikazu Takei, Junko Sayama, Yusuke Yamada, Shinji Kaneko, Mika K. Kato, Yukinari Biochem Biophys Rep Research Article Sensitive and specific monoclonal antibodies (mAbs) targeting podoplanin (PDPN) are needed for immunohistochemical analyses using formalin-fixed paraffin-embedded tissues because PDPN is known as a lymphatic endothelial cell maker in pathology. Recently, we established anti-PDPN mAbs against many species, such as human, mouse, rat, rabbit, dog, cat, bovine, pig, horse, goat, tiger, alpaca, and Tasmanian devil. However, anti-bear PDPN (bPDPN) has not been established yet. In this study, we immunized mice with bPDPN-overexpressing Chinese hamster ovary (CHO)-K1 (CHO/bPDPN) cells, and screened mAbs against bPDPN using flow cytometry. One of the mAbs, PMab-247 (IgG(1), kappa), specifically detected CHO/bPDPN cells by flow cytometry and immunohistochemistry. Our findings suggest the potential usefulness of PMab-247 for the functional analyses of bPDPN. Elsevier 2019-04-28 /pmc/articles/PMC6488525/ /pubmed/31061899 http://dx.doi.org/10.1016/j.bbrep.2019.100644 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Furusawa, Yoshikazu
Takei, Junko
Sayama, Yusuke
Yamada, Shinji
Kaneko, Mika K.
Kato, Yukinari
Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title_full Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title_fullStr Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title_full_unstemmed Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title_short Development of an anti-bear podoplanin monoclonal antibody PMab-247 for immunohistochemical analysis
title_sort development of an anti-bear podoplanin monoclonal antibody pmab-247 for immunohistochemical analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488525/
https://www.ncbi.nlm.nih.gov/pubmed/31061899
http://dx.doi.org/10.1016/j.bbrep.2019.100644
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