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Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9

The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to an RNA guide. Unlike other Cas9 orthologs, Corynebacterium diphtheriae Cas9 (CdCas9) recognizes the promiscuous NNRHHHY PAM. However, the CdCas9-mediated PAM rec...

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Detalles Bibliográficos
Autores principales:	Hirano, Seiichi, Abudayyeh, Omar O., Gootenberg, Jonathan S., Horii, Takuro, Ishitani, Ryuichiro, Hatada, Izuho, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488586/ https://www.ncbi.nlm.nih.gov/pubmed/31036811 http://dx.doi.org/10.1038/s41467-019-09741-6

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