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Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice

Fumigaclavine C (FC), an active indole alkaloid, is obtained from endophytic Aspergillus terreus (strain No. FC118) by the root of Rhizophora stylosa (Rhizophoraceae). This study is designed to evaluate whether FC has anti-adipogenic effects in 3T3-L1 adipocytes and whether it ameliorates lipid accu...

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Autores principales: Yu, Wan-Guo, He, Yun, Chen, Yun-Fang, Gao, Xiao-Yao, Ning, Wan-E, Liu, Chun-You, Tang, Ting-Fan, Liu, Quan, Huang, Xiao-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Physiological Society and The Korean Society of Pharmacology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488706/
https://www.ncbi.nlm.nih.gov/pubmed/31080347
http://dx.doi.org/10.4196/kjpp.2019.23.3.161
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author Yu, Wan-Guo
He, Yun
Chen, Yun-Fang
Gao, Xiao-Yao
Ning, Wan-E
Liu, Chun-You
Tang, Ting-Fan
Liu, Quan
Huang, Xiao-Cheng
author_facet Yu, Wan-Guo
He, Yun
Chen, Yun-Fang
Gao, Xiao-Yao
Ning, Wan-E
Liu, Chun-You
Tang, Ting-Fan
Liu, Quan
Huang, Xiao-Cheng
author_sort Yu, Wan-Guo
collection PubMed
description Fumigaclavine C (FC), an active indole alkaloid, is obtained from endophytic Aspergillus terreus (strain No. FC118) by the root of Rhizophora stylosa (Rhizophoraceae). This study is designed to evaluate whether FC has anti-adipogenic effects in 3T3-L1 adipocytes and whether it ameliorates lipid accumulation in high-fat diet (HFD)-induced obese mice. FC notably increased the levels of glycerol in the culture supernatants and markedly reduced lipid accumulation in 3T3-L1 adipocytes. FC differentially inhibited the expressions of adipogenesis-related genes, including the peroxisome proliferator-activated receptor proteins, CCAAT/enhancer-binding proteins, and sterol regulatory element-binding proteins. FC markedly reduced the expressions of lipid synthesis-related genes, such as the fatty acid binding protein, lipoprotein lipase, and fatty acid synthase. Furthermore, FC significantly increased the expressions of lipolysis-related genes, such as the hormone-sensitive lipase, Aquaporin-7, and adipose triglyceride lipase. In HFD-induced obese mice, intraperitoneal injections of FC decreased both the body weight and visceral adipose tissue weight. FC administration significantly reduced lipid accumulation. Moreover, FC could dose-dependently and differentially regulate the expressions of lipid metabolism-related transcription factors. All these data indicated that FC exhibited anti-obesity effects through modulating adipogenesis and lipolysis.
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spelling pubmed-64887062019-05-10 Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice Yu, Wan-Guo He, Yun Chen, Yun-Fang Gao, Xiao-Yao Ning, Wan-E Liu, Chun-You Tang, Ting-Fan Liu, Quan Huang, Xiao-Cheng Korean J Physiol Pharmacol Original Article Fumigaclavine C (FC), an active indole alkaloid, is obtained from endophytic Aspergillus terreus (strain No. FC118) by the root of Rhizophora stylosa (Rhizophoraceae). This study is designed to evaluate whether FC has anti-adipogenic effects in 3T3-L1 adipocytes and whether it ameliorates lipid accumulation in high-fat diet (HFD)-induced obese mice. FC notably increased the levels of glycerol in the culture supernatants and markedly reduced lipid accumulation in 3T3-L1 adipocytes. FC differentially inhibited the expressions of adipogenesis-related genes, including the peroxisome proliferator-activated receptor proteins, CCAAT/enhancer-binding proteins, and sterol regulatory element-binding proteins. FC markedly reduced the expressions of lipid synthesis-related genes, such as the fatty acid binding protein, lipoprotein lipase, and fatty acid synthase. Furthermore, FC significantly increased the expressions of lipolysis-related genes, such as the hormone-sensitive lipase, Aquaporin-7, and adipose triglyceride lipase. In HFD-induced obese mice, intraperitoneal injections of FC decreased both the body weight and visceral adipose tissue weight. FC administration significantly reduced lipid accumulation. Moreover, FC could dose-dependently and differentially regulate the expressions of lipid metabolism-related transcription factors. All these data indicated that FC exhibited anti-obesity effects through modulating adipogenesis and lipolysis. The Korean Physiological Society and The Korean Society of Pharmacology 2019-05 2019-04-24 /pmc/articles/PMC6488706/ /pubmed/31080347 http://dx.doi.org/10.4196/kjpp.2019.23.3.161 Text en Copyright © Korean J Physiol Pharmacol http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Yu, Wan-Guo
He, Yun
Chen, Yun-Fang
Gao, Xiao-Yao
Ning, Wan-E
Liu, Chun-You
Tang, Ting-Fan
Liu, Quan
Huang, Xiao-Cheng
Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title_full Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title_fullStr Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title_full_unstemmed Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title_short Fumigaclavine C attenuates adipogenesis in 3T3-L1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
title_sort fumigaclavine c attenuates adipogenesis in 3t3-l1 adipocytes and ameliorates lipid accumulation in high-fat diet-induced obese mice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488706/
https://www.ncbi.nlm.nih.gov/pubmed/31080347
http://dx.doi.org/10.4196/kjpp.2019.23.3.161
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