CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B...

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Autores principales: Butt, Haroon, Eid, Ayman, Momin, Afaque A., Bazin, Jeremie, Crespi, Martin, Arold, Stefan T., Mahfouz, Magdy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489355/
https://www.ncbi.nlm.nih.gov/pubmed/31036069
http://dx.doi.org/10.1186/s13059-019-1680-9
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author Butt, Haroon
Eid, Ayman
Momin, Afaque A.
Bazin, Jeremie
Crespi, Martin
Arold, Stefan T.
Mahfouz, Magdy M.
author_facet Butt, Haroon
Eid, Ayman
Momin, Afaque A.
Bazin, Jeremie
Crespi, Martin
Arold, Stefan T.
Mahfouz, Magdy M.
author_sort Butt, Haroon
collection PubMed
description Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1680-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-64893552019-05-08 CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors Butt, Haroon Eid, Ayman Momin, Afaque A. Bazin, Jeremie Crespi, Martin Arold, Stefan T. Mahfouz, Magdy M. Genome Biol Method Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B1 mutant variants, termed SF3B1-GEX1A-Resistant (SGR), confer variable levels of resistance to splicing inhibitors. Studies of the structural basis of the splicing inhibitor binding to SGRs corroborate the resistance phenotype. This directed evolution platform can be used to interrogate and evolve the molecular functions of key biomolecules and to engineer crop traits for improved performance and adaptation under climate change conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13059-019-1680-9) contains supplementary material, which is available to authorized users. BioMed Central 2019-04-30 /pmc/articles/PMC6489355/ /pubmed/31036069 http://dx.doi.org/10.1186/s13059-019-1680-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Method
Butt, Haroon
Eid, Ayman
Momin, Afaque A.
Bazin, Jeremie
Crespi, Martin
Arold, Stefan T.
Mahfouz, Magdy M.
CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title_full CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title_fullStr CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title_full_unstemmed CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title_short CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors
title_sort crispr directed evolution of the spliceosome for resistance to splicing inhibitors
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489355/
https://www.ncbi.nlm.nih.gov/pubmed/31036069
http://dx.doi.org/10.1186/s13059-019-1680-9
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