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CRISPR directed evolution of the spliceosome for resistance to splicing inhibitors

Increasing genetic diversity via directed evolution holds great promise to accelerate trait development and crop improvement. We developed a CRISPR/Cas-based directed evolution platform in plants to evolve the rice (Oryza sativa) SF3B1 spliceosomal protein for resistance to splicing inhibitors. SF3B...

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Detalles Bibliográficos
Autores principales:	Butt, Haroon, Eid, Ayman, Momin, Afaque A., Bazin, Jeremie, Crespi, Martin, Arold, Stefan T., Mahfouz, Magdy M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2019
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489355/ https://www.ncbi.nlm.nih.gov/pubmed/31036069 http://dx.doi.org/10.1186/s13059-019-1680-9

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