Measuring sequencer size bias using REcount: a novel method for highly accurate Illumina sequencing-based quantification

Quantification of DNA sequence tags from engineered constructs such as plasmids, transposons, or other transgenes underlies many functional genomics measurements. Typically, such measurements rely on PCR followed by next-generation sequencing. However, PCR amplification can introduce significant qua...

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Detalles Bibliográficos
Autores principales: Gohl, Daryl M., Magli, Alessandro, Garbe, John, Becker, Aaron, Johnson, Darrell M., Anderson, Shea, Auch, Benjamin, Billstein, Bradley, Froehling, Elyse, McDevitt, Shana L., Beckman, Kenneth B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6489363/
https://www.ncbi.nlm.nih.gov/pubmed/31036053
http://dx.doi.org/10.1186/s13059-019-1691-6

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