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Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method

BACKGROUND: In this study, bacteriostatic potency of the Iron oxide nanoparticles against Enterococcus faecalis (E. faecalis) (a clinical sample and the ATCC11700 strain) was investigated. METHODS: Nanoparticles’ bacteriostatic concentration was determined and used to appraise the characteristics of...

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Autores principales: Shahbazi, Erfan, Morshedzadeh, Firouzeh, Zaeifi, Davood
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490414/
https://www.ncbi.nlm.nih.gov/pubmed/31057720
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author Shahbazi, Erfan
Morshedzadeh, Firouzeh
Zaeifi, Davood
author_facet Shahbazi, Erfan
Morshedzadeh, Firouzeh
Zaeifi, Davood
author_sort Shahbazi, Erfan
collection PubMed
description BACKGROUND: In this study, bacteriostatic potency of the Iron oxide nanoparticles against Enterococcus faecalis (E. faecalis) (a clinical sample and the ATCC11700 strain) was investigated. METHODS: Nanoparticles’ bacteriostatic concentration was determined and used to appraise the characteristics of the Iron Oxide (Fe(2)O(3)) against the isolates. Antimicrobial examinations with 10(8 )cfu.ml(−1) were performed at the baseline. Due to evaluation level of potency, after performing Minimum Inhibitory Concentration (MIC), the assessment of death kinetic and susceptibility constant of nanoparticles was done by suspension at two MICs in 0 to 360 min treatment time. RESULTS: Fe(2)O(3) nanoparticles in size range of 10–50 nm demonstrated the most effective susceptibility reaction against E. faecalis and ATCC11700 strain in Z=78.125 ml/μg( −1) and 39.0625 ml/μg( −1), respectively. The kinetic reaction of E. faecalis against Fe(2)O(3) suspension was supposed to be decreased through the elapse of treatment time, whereas increased concentration was along with bacteria growth after a certain time. So, the efficient concentration of nanoparticles was applied with semi-sensitive and antibiotic resistant for both strains. However, synergism of Fe(2)O(3) nanoparticles with Ceftazidime and Clindamycin revealed a higher susceptibility compared with Fe(2)O(3)nanoparticles alone against E. faecalis. CONCLUSION: The experimental results reveal that Fe(2)O(3) has a strong antimicrobial effect at a certain concentration over the time so could potentially be used for bacterial inhibition and this feature will be strengthened in combination with antibiotics.
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spelling pubmed-64904142019-05-03 Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method Shahbazi, Erfan Morshedzadeh, Firouzeh Zaeifi, Davood Avicenna J Med Biotechnol Original Article BACKGROUND: In this study, bacteriostatic potency of the Iron oxide nanoparticles against Enterococcus faecalis (E. faecalis) (a clinical sample and the ATCC11700 strain) was investigated. METHODS: Nanoparticles’ bacteriostatic concentration was determined and used to appraise the characteristics of the Iron Oxide (Fe(2)O(3)) against the isolates. Antimicrobial examinations with 10(8 )cfu.ml(−1) were performed at the baseline. Due to evaluation level of potency, after performing Minimum Inhibitory Concentration (MIC), the assessment of death kinetic and susceptibility constant of nanoparticles was done by suspension at two MICs in 0 to 360 min treatment time. RESULTS: Fe(2)O(3) nanoparticles in size range of 10–50 nm demonstrated the most effective susceptibility reaction against E. faecalis and ATCC11700 strain in Z=78.125 ml/μg( −1) and 39.0625 ml/μg( −1), respectively. The kinetic reaction of E. faecalis against Fe(2)O(3) suspension was supposed to be decreased through the elapse of treatment time, whereas increased concentration was along with bacteria growth after a certain time. So, the efficient concentration of nanoparticles was applied with semi-sensitive and antibiotic resistant for both strains. However, synergism of Fe(2)O(3) nanoparticles with Ceftazidime and Clindamycin revealed a higher susceptibility compared with Fe(2)O(3)nanoparticles alone against E. faecalis. CONCLUSION: The experimental results reveal that Fe(2)O(3) has a strong antimicrobial effect at a certain concentration over the time so could potentially be used for bacterial inhibition and this feature will be strengthened in combination with antibiotics. Avicenna Research Institute 2019 /pmc/articles/PMC6490414/ /pubmed/31057720 Text en Copyright© 2019 Avicenna Research Institute http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Shahbazi, Erfan
Morshedzadeh, Firouzeh
Zaeifi, Davood
Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title_full Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title_fullStr Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title_full_unstemmed Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title_short Bacteriostatic Potency of Fe(2)O(3) Against Enterococcus faecalis in Synergy with Antibiotics by DDST Method
title_sort bacteriostatic potency of fe(2)o(3) against enterococcus faecalis in synergy with antibiotics by ddst method
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490414/
https://www.ncbi.nlm.nih.gov/pubmed/31057720
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