Cargando…

Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016

BACKGROUND: Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimat...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Xin, Zeng, Mei, Xu, Jialiang, Zhou, Haijian, Gu, Baoke, Li, Zhenpeng, Jin, Huiming, Wang, Xiaoxun, Zhang, Wen, Hu, Yongfei, Xiao, Wenjia, Zhu, Baoli, Xu, Xuebin, Kan, Biao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491383/
https://www.ncbi.nlm.nih.gov/pubmed/30905850
http://dx.doi.org/10.1016/j.ebiom.2019.03.006
_version_ 1783414923603214336
author Lu, Xin
Zeng, Mei
Xu, Jialiang
Zhou, Haijian
Gu, Baoke
Li, Zhenpeng
Jin, Huiming
Wang, Xiaoxun
Zhang, Wen
Hu, Yongfei
Xiao, Wenjia
Zhu, Baoli
Xu, Xuebin
Kan, Biao
author_facet Lu, Xin
Zeng, Mei
Xu, Jialiang
Zhou, Haijian
Gu, Baoke
Li, Zhenpeng
Jin, Huiming
Wang, Xiaoxun
Zhang, Wen
Hu, Yongfei
Xiao, Wenjia
Zhu, Baoli
Xu, Xuebin
Kan, Biao
author_sort Lu, Xin
collection PubMed
description BACKGROUND: Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated. METHODS: This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes. FINDINGS: Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5 years. All strains, including fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing strains, were multi-drug resistant. S. Typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. Phylogeny based on the genomes combined with complete plasmid sequences revealed some clusters, suggesting the presence of mcr-1-harbouring Salmonella outbreaks in the community. Most mcr-1-positive strains were clustered together with the pork strains, strongly suggesting pork consumption as a main infection source. INTERPRETATION: The mcr-1-harbouring Salmonella prevalence in community-acquired diarrhoea displays a rapid increase trend, and the ESBL-mcr-1-harbouring Salmonella poses a threat for children. These findings highlight the necessary and significance of prohibiting colistin use in animals and continuous monitoring of mcr-1-harbouring Salmonella.
format Online
Article
Text
id pubmed-6491383
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-64913832019-05-06 Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016 Lu, Xin Zeng, Mei Xu, Jialiang Zhou, Haijian Gu, Baoke Li, Zhenpeng Jin, Huiming Wang, Xiaoxun Zhang, Wen Hu, Yongfei Xiao, Wenjia Zhu, Baoli Xu, Xuebin Kan, Biao EBioMedicine Research paper BACKGROUND: Colistin resistance mediated by mcr-1-harbouring plasmids is an emerging threat in Enterobacteriaceae, like Salmonella. Based on its major contribution to the diarrhoea burden, the epidemic state and threat of mcr-1-harbouring Salmonella in community-acquired infections should be estimated. METHODS: This retrospective study analysed the mcr-1 gene incidence in Salmonella strains collected from a surveillance on diarrhoeal outpatients in Shanghai Municipality, China, 2006–2016. Molecular characteristics of the mcr-1-positive strains and their plasmids were determined by genome sequencing. The transfer abilities of these plasmids were measured with various conjugation strains, species, and serotypes. FINDINGS: Among the 12,053 Salmonella isolates, 37 mcr-1-harbouring strains, in which 35 were serovar Typhimurium, were detected first in 2012 and with increasing frequency after 2015. Most patients infected with mcr-1-harbouring strains were aged <5 years. All strains, including fluoroquinolone-resistant and/or extended-spectrum β-lactamase-producing strains, were multi-drug resistant. S. Typhimurium had higher mcr-1 plasmid acquisition ability compared with other common serovars. Phylogeny based on the genomes combined with complete plasmid sequences revealed some clusters, suggesting the presence of mcr-1-harbouring Salmonella outbreaks in the community. Most mcr-1-positive strains were clustered together with the pork strains, strongly suggesting pork consumption as a main infection source. INTERPRETATION: The mcr-1-harbouring Salmonella prevalence in community-acquired diarrhoea displays a rapid increase trend, and the ESBL-mcr-1-harbouring Salmonella poses a threat for children. These findings highlight the necessary and significance of prohibiting colistin use in animals and continuous monitoring of mcr-1-harbouring Salmonella. Elsevier 2019-03-21 /pmc/articles/PMC6491383/ /pubmed/30905850 http://dx.doi.org/10.1016/j.ebiom.2019.03.006 Text en © 2019 The Authors. Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research paper
Lu, Xin
Zeng, Mei
Xu, Jialiang
Zhou, Haijian
Gu, Baoke
Li, Zhenpeng
Jin, Huiming
Wang, Xiaoxun
Zhang, Wen
Hu, Yongfei
Xiao, Wenjia
Zhu, Baoli
Xu, Xuebin
Kan, Biao
Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title_full Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title_fullStr Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title_full_unstemmed Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title_short Epidemiologic and genomic insights on mcr-1-harbouring Salmonella from diarrhoeal outpatients in Shanghai, China, 2006–2016
title_sort epidemiologic and genomic insights on mcr-1-harbouring salmonella from diarrhoeal outpatients in shanghai, china, 2006–2016
topic Research paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491383/
https://www.ncbi.nlm.nih.gov/pubmed/30905850
http://dx.doi.org/10.1016/j.ebiom.2019.03.006
work_keys_str_mv AT luxin epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT zengmei epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT xujialiang epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT zhouhaijian epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT gubaoke epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT lizhenpeng epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT jinhuiming epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT wangxiaoxun epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT zhangwen epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT huyongfei epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT xiaowenjia epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT zhubaoli epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT xuxuebin epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016
AT kanbiao epidemiologicandgenomicinsightsonmcr1harbouringsalmonellafromdiarrhoealoutpatientsinshanghaichina20062016