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Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions

Multiple sclerosis (MS) is a demyelinating autoimmune disease in which innate and adaptive immune cells infiltrate the central nervous system (CNS) and damage the myelin sheaths surrounding the axons. Upon activation, infiltrated macrophages, CNS-resident microglia, and astrocytes switch their metab...

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Autores principales: Wetzels, Suzan, Vanmierlo, Tim, Scheijen, Jean L. J. M., van Horssen, Jack, Amor, Sandra, Somers, Veerle, Schalkwijk, Casper G., Hendriks, Jerome J. A., Wouters, Kristiaan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491451/
https://www.ncbi.nlm.nih.gov/pubmed/31068938
http://dx.doi.org/10.3389/fimmu.2019.00855
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author Wetzels, Suzan
Vanmierlo, Tim
Scheijen, Jean L. J. M.
van Horssen, Jack
Amor, Sandra
Somers, Veerle
Schalkwijk, Casper G.
Hendriks, Jerome J. A.
Wouters, Kristiaan
author_facet Wetzels, Suzan
Vanmierlo, Tim
Scheijen, Jean L. J. M.
van Horssen, Jack
Amor, Sandra
Somers, Veerle
Schalkwijk, Casper G.
Hendriks, Jerome J. A.
Wouters, Kristiaan
author_sort Wetzels, Suzan
collection PubMed
description Multiple sclerosis (MS) is a demyelinating autoimmune disease in which innate and adaptive immune cells infiltrate the central nervous system (CNS) and damage the myelin sheaths surrounding the axons. Upon activation, infiltrated macrophages, CNS-resident microglia, and astrocytes switch their metabolism toward glycolysis, resulting in the formation of α-dicarbonyls, such as methylglyoxal (MGO) and glyoxal (GO). These potent glycating agents lead to the formation of advanced glycation endproducts (AGEs) after reaction with amino acids. We hypothesize that AGE levels are increased in MS lesions due to the inflammatory activation of macrophages and astrocytes. First, we measured tissue levels of AGEs in brain samples of MS patients and controls. Analysis of MS patient and non-demented control (NDC) specimens showed a significant increase in protein-bound N(δ)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1), the major AGE, compared to white matter of NDCs (107 ± 11 vs. 154 ± 21, p < 0.05). In addition, immunohistochemistry revealed that MGO-derived AGEs were specifically present in astrocytes, whereas the receptor for AGEs, RAGE, was detected on microglia/macrophages. Moreover, in cerebrospinal fluid from MS patients, α-dicarbonyls and free AGEs correlated with their respective levels in the plasma, whereas this was not observed for protein-bound AGEs. Taken together, our data show that MG-H1 is produced by astrocytes. This suggests that AGEs secreted by astrocytes have paracrine effects on RAGE-positive macrophages/microglia and thereby contribute to the pathology of MS.
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spelling pubmed-64914512019-05-08 Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions Wetzels, Suzan Vanmierlo, Tim Scheijen, Jean L. J. M. van Horssen, Jack Amor, Sandra Somers, Veerle Schalkwijk, Casper G. Hendriks, Jerome J. A. Wouters, Kristiaan Front Immunol Immunology Multiple sclerosis (MS) is a demyelinating autoimmune disease in which innate and adaptive immune cells infiltrate the central nervous system (CNS) and damage the myelin sheaths surrounding the axons. Upon activation, infiltrated macrophages, CNS-resident microglia, and astrocytes switch their metabolism toward glycolysis, resulting in the formation of α-dicarbonyls, such as methylglyoxal (MGO) and glyoxal (GO). These potent glycating agents lead to the formation of advanced glycation endproducts (AGEs) after reaction with amino acids. We hypothesize that AGE levels are increased in MS lesions due to the inflammatory activation of macrophages and astrocytes. First, we measured tissue levels of AGEs in brain samples of MS patients and controls. Analysis of MS patient and non-demented control (NDC) specimens showed a significant increase in protein-bound N(δ)-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine (MG-H1), the major AGE, compared to white matter of NDCs (107 ± 11 vs. 154 ± 21, p < 0.05). In addition, immunohistochemistry revealed that MGO-derived AGEs were specifically present in astrocytes, whereas the receptor for AGEs, RAGE, was detected on microglia/macrophages. Moreover, in cerebrospinal fluid from MS patients, α-dicarbonyls and free AGEs correlated with their respective levels in the plasma, whereas this was not observed for protein-bound AGEs. Taken together, our data show that MG-H1 is produced by astrocytes. This suggests that AGEs secreted by astrocytes have paracrine effects on RAGE-positive macrophages/microglia and thereby contribute to the pathology of MS. Frontiers Media S.A. 2019-04-24 /pmc/articles/PMC6491451/ /pubmed/31068938 http://dx.doi.org/10.3389/fimmu.2019.00855 Text en Copyright © 2019 Wetzels, Vanmierlo, Scheijen, van Horssen, Amor, Somers, Schalkwijk, Hendriks and Wouters. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wetzels, Suzan
Vanmierlo, Tim
Scheijen, Jean L. J. M.
van Horssen, Jack
Amor, Sandra
Somers, Veerle
Schalkwijk, Casper G.
Hendriks, Jerome J. A.
Wouters, Kristiaan
Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title_full Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title_fullStr Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title_full_unstemmed Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title_short Methylglyoxal-Derived Advanced Glycation Endproducts Accumulate in Multiple Sclerosis Lesions
title_sort methylglyoxal-derived advanced glycation endproducts accumulate in multiple sclerosis lesions
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491451/
https://www.ncbi.nlm.nih.gov/pubmed/31068938
http://dx.doi.org/10.3389/fimmu.2019.00855
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