Cargando…

Automated Segmentation of Fluorescence Microscopy Images for 3D Cell Detection in human-derived Cardiospheres

The ‘cardiosphere’ is a 3D cluster of cardiac progenitor cells recapitulating a stem cell niche-like microenvironment with a potential for disease and regeneration modelling of the failing human myocardium. In this multicellular 3D context, it is extremely important to decrypt the spatial distributi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Salvi, Massimo, Morbiducci, Umberto, Amadeo, Francesco, Santoro, Rosaria, Angelini, Francesco, Chimenti, Isotta, Massai, Diana, Messina, Elisa, Giacomello, Alessandro, Pesce, Maurizio, Molinari, Filippo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491482/ https://www.ncbi.nlm.nih.gov/pubmed/31040327 http://dx.doi.org/10.1038/s41598-019-43137-2

Ejemplares similares

Serum and supplement optimization for EU GMP-compliance in cardiospheres cell culture
por: Chimenti, Isotta, et al.
Publicado: (2014)

Cardiospheres and tissue engineering for myocardial regeneration: potential for clinical application
por: Gaetani, Roberto, et al.
Publicado: (2010)

Normal versus Pathological Cardiac Fibroblast-Derived Extracellular Matrix Differentially Modulates Cardiosphere-Derived Cell Paracrine Properties and Commitment
por: Pagano, Francesca, et al.
Publicado: (2017)

Β-blockers treatment of cardiac surgery patients enhances isolation and improves phenotype of cardiosphere-derived cells
por: Chimenti, Isotta, et al.
Publicado: (2016)

EMT/MET at the Crossroad of Stemness, Regeneration and Oncogenesis: The Ying-Yang Equilibrium Recapitulated in Cell Spheroids
por: Forte, Elvira, et al.
Publicado: (2017)

Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells
por: D'Elia, Piera, et al.
Publicado: (2013)

Cardiosphere-Derived Cells Improve Function in the Infarcted Rat Heart for at Least 16 Weeks – an MRI Study
por: Carr, Carolyn A., et al.
Publicado: (2011)

When Stiffness Matters: Mechanosensing in Heart Development and Disease
por: Gaetani, Roberto, et al.
Publicado: (2020)

Getting Old through the Blood: Circulating Molecules in Aging and Senescence of Cardiovascular Regenerative Cells
por: Angelini, Francesco, et al.
Publicado: (2017)

Image-Based Three-Dimensional Analysis to Characterize the Texture of Porous Scaffolds
por: Massai, Diana, et al.
Publicado: (2014)

Metabolic maturation of differentiating cardiosphere-derived cells
por: Pakzad, Khadijeh Kathy, et al.
Publicado: (2021)

An automated 3D-printed perfusion bioreactor combinable with pulsed electromagnetic field stimulators for bone tissue investigations
por: Gabetti, Stefano, et al.
Publicado: (2022)

Notch pathway activation enhances cardiosphere in vitro expansion
por: Secco, Ilaria, et al.
Publicado: (2018)

Bone marrow-derived cells can acquire cardiac stem cells properties in damaged heart
por: Barile, Lucio, et al.
Publicado: (2011)

Foetal bovine serum-derived exosomes affect yield and phenotype of human cardiac progenitor cell culture
por: Angelini, Francesco, et al.
Publicado: (2016)

Cell Shape and Cardiosphere Differentiation: A Revelation by Proteomic Profiling
por: Kawaguchi, Nanako, et al.
Publicado: (2013)

Human Cardiospheres as a Source of Multipotent Stem and Progenitor Cells
por: Barile, Lucio, et al.
Publicado: (2013)

Cardiosphere-Derived Cells Require Endoglin for Paracrine-Mediated Angiogenesis
por: Redgrave, Rachael E., et al.
Publicado: (2017)

Building an Artificial Cardiac Microenvironment: A Focus on the Extracellular Matrix
por: Pagliarosi, Olivia, et al.
Publicado: (2020)

Fast and accurate automated cell boundary determination for fluorescence microscopy
por: Arce, Stephen Hugo, et al.
Publicado: (2013)

Cardiosphere-Derived Cells Demonstrate Metabolic Flexibility That Is Influenced by Adhesion Status
por: Afzal, Junaid, et al.
Publicado: (2017)

2194: Effects of anoxia on viability and differentiation of human cardiosphere-derived cells
por: Khanjyan, Michael, et al.
Publicado: (2018)

Automated detection of fluorescent cells in in‐resin fluorescence sections for integrated light and electron microscopy
por: DELPIANO, J., et al.
Publicado: (2018)

A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
por: Laurino, Annunziatina, et al.
Publicado: (2023)

Human Lung Spheroids as In Vitro Niches of Lung Progenitor Cells with Distinctive Paracrine and Plasticity Properties
por: Chimenti, Isotta, et al.
Publicado: (2016)

Validation of the Cardiosphere Method to Culture Cardiac Progenitor Cells from Myocardial Tissue
por: Davis, Darryl R., et al.
Publicado: (2009)

Cardiac regenerative potential of cardiosphere-derived cells from adult dog hearts
por: Hensley, Michael Taylor, et al.
Publicado: (2015)

Cardiosphere-derived cells do not improve cardiac function in rats with cardiac failure
por: Kasai-Brunswick, Taís Hanae, et al.
Publicado: (2017)

Therapeutic benefits of intravenous cardiosphere-derived cell therapy in rats with pulmonary hypertension
por: Middleton, Ryan C., et al.
Publicado: (2017)

Automated Analysis of Fluorescence Microscopy Images to Identify Protein-Protein Interactions
por: Venkatraman, S., et al.
Publicado: (2006)

Stochastic Micro-Pattern for Automated Correlative Fluorescence - Scanning Electron Microscopy
por: Begemann, Isabell, et al.
Publicado: (2015)

Infection Counter: Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy
por: Culley, Siân, et al.
Publicado: (2016)

Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish
por: Logan, Savannah L., et al.
Publicado: (2018)

ACDC: Automated Cell Detection and Counting for Time-Lapse Fluorescence Microscopy
por: Rundo, Leonardo, et al.
Publicado: (2020)

Cardiac Progenitor Cells: The Matrix Has You
por: Castaldo, Clotilde, et al.
Publicado: (2018)

3D Cultures for Modelling the Microenvironment: Current Research Trends and Applications
por: Gaetani, Roberto, et al.
Publicado: (2023)

Dose-dependent functional benefit of human cardiosphere transplantation in mice with acute myocardial infarction
por: Shen, Deliang, et al.
Publicado: (2012)

Exosomes Secreted by Normoxic and Hypoxic Cardiosphere-derived Cells Have Anti-apoptotic Effect
por: Namazi, Helia, et al.
Publicado: (2018)

Intracoronary allogeneic cardiosphere‐derived stem cells are safe for use in dogs with dilated cardiomyopathy
por: Hensley, Michael Taylor, et al.
Publicado: (2017)

Uncovering the molecular identity of cardiosphere-derived cells (CDCs) by single-cell RNA sequencing
por: Kogan, Palgit-S., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_8f0a3kakm9vmnep7dtamt4o6l3