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CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement

CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture productivity, but the possibility of unintended off-target edits has evoked some concerns. Here we employ a three-step strategy to investigate Cas9 nuclease specificity in a complex plant genome. Our approach pairs...

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Detalles Bibliográficos
Autores principales:	Young, Joshua, Zastrow-Hayes, Gina, Deschamps, Stéphane, Svitashev, Sergei, Zaremba, Mindaugas, Acharya, Ananta, Paulraj, Sushmitha, Peterson-Burch, Brooke, Schwartz, Chris, Djukanovic, Vesna, Lenderts, Brian, Feigenbutz, Lanie, Wang, Lijuan, Alarcon, Clara, Siksnys, Virginijus, May, Gregory, Chilcoat, N. Doane, Kumar, Sandeep
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491584/ https://www.ncbi.nlm.nih.gov/pubmed/31040331 http://dx.doi.org/10.1038/s41598-019-43141-6

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