Cargando…

CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement

CRISPR-Cas9 enabled genome engineering has great potential for improving agriculture productivity, but the possibility of unintended off-target edits has evoked some concerns. Here we employ a three-step strategy to investigate Cas9 nuclease specificity in a complex plant genome. Our approach pairs...

Descripción completa

Detalles Bibliográficos
Autores principales:	Young, Joshua, Zastrow-Hayes, Gina, Deschamps, Stéphane, Svitashev, Sergei, Zaremba, Mindaugas, Acharya, Ananta, Paulraj, Sushmitha, Peterson-Burch, Brooke, Schwartz, Chris, Djukanovic, Vesna, Lenderts, Brian, Feigenbutz, Lanie, Wang, Lijuan, Alarcon, Clara, Siksnys, Virginijus, May, Gregory, Chilcoat, N. Doane, Kumar, Sandeep
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491584/ https://www.ncbi.nlm.nih.gov/pubmed/31040331 http://dx.doi.org/10.1038/s41598-019-43141-6

Ejemplares similares

Miniature type V-F CRISPR-Cas nucleases enable targeted DNA modification in cells
por: Bigelyte, Greta, et al.
Publicado: (2021)

Advances in Agrobacterium transformation and vector design result in high‐frequency targeted gene insertion in maize
por: Peterson, Dave, et al.
Publicado: (2021)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

Crystal structure of the R-protein of the multisubunit ATP-dependent restriction endonuclease NgoAVII
por: Tamulaitiene, Giedre, et al.
Publicado: (2014)

How PspGI, catalytic domain of EcoRII and Ecl18kI acquire specificities for different DNA targets
por: Tamulaitis, Gintautas, et al.
Publicado: (2008)

Nucleotide flipping by restriction enzymes analyzed by 2-aminopurine steady-state fluorescence
por: Tamulaitis, Gintautas, et al.
Publicado: (2007)

CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases
por: Toliusis, Paulius, et al.
Publicado: (2017)

Targeted mutagenesis of a conserved anther‐expressed P450 gene confers male sterility in monocots
por: Cigan, A. Mark, et al.
Publicado: (2016)

Structural mechanisms of the degenerate sequence recognition by Bse634I restriction endonuclease
por: Manakova, Elena, et al.
Publicado: (2012)

Functional significance of protein assemblies predicted by the crystal structure of the restriction endonuclease BsaWI
por: Tamulaitis, Gintautas, et al.
Publicado: (2015)

Complex Trait Loci in Maize Enabled by CRISPR-Cas9 Mediated Gene Insertion
por: Gao, Huirong, et al.
Publicado: (2020)

Structural insight into the specificity of the B3 DNA-binding domains provided by the co-crystal structure of the C-terminal fragment of BfiI restriction enzyme
por: Golovenko, Dmitrij, et al.
Publicado: (2014)

A novel mechanism for the scission of double-stranded DNA: BfiI cuts both 3′–5′ and 5′–3′ strands by rotating a single active site
por: Sasnauskas, Giedrius, et al.
Publicado: (2010)

DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis
por: Zaremba, Mindaugas, et al.
Publicado: (2014)

A catalogue of biochemically diverse CRISPR-Cas9 orthologs
por: Gasiunas, Giedrius, et al.
Publicado: (2020)

Central base pair flipping and discrimination by PspGI
por: Szczepanowski, Roman H., et al.
Publicado: (2008)

Restriction endonuclease AgeI is a monomer which dimerizes to cleave DNA
por: Tamulaitiene, Giedre, et al.
Publicado: (2017)

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
por: Svitashev, Sergei, et al.
Publicado: (2016)

Chromatin loop anchors contain core structural components of the gene expression machinery in maize
por: Deschamps, Stéphane, et al.
Publicado: (2021)

A new family of CRISPR‐type V nucleases with C‐rich PAM recognition
por: Urbaitis, Tomas, et al.
Publicado: (2022)

The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor
por: Toliusis, Paulius, et al.
Publicado: (2018)

High efficiency Agrobacterium‐mediated site‐specific gene integration in maize utilizing the FLP‐FRT recombination system
por: Anand, Ajith, et al.
Publicado: (2019)

In Vivo Analysis of the Major Exocytosis-sensitive Phosphoprotein in Tetrahymena
por: Chilcoat, N. Doane, et al.
Publicado: (1997)

Molecular mechanisms of CRISPR-mediated microbial immunity
por: Gasiunas, Giedrius, et al.
Publicado: (2013)

Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1
por: Sasnauskas, Giedrius, et al.
Publicado: (2018)

DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme
por: Zaremba, Mindaugas, et al.
Publicado: (2010)

Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation
por: Sasnauskas, Giedrius, et al.
Publicado: (2011)

Degenerate sequence recognition by the monomeric restriction enzyme: single mutation converts BcnI into a strand-specific nicking endonuclease
por: Kostiuk, Georgij, et al.
Publicado: (2011)

The recognition domain of the methyl-specific endonuclease McrBC flips out 5-methylcytosine
por: Sukackaite, Rasa, et al.
Publicado: (2012)

Template-directed addition of nucleosides to DNA by the BfiI restriction enzyme
por: Sasnauskas, Giedrius, et al.
Publicado: (2008)

Structural mechanisms for the 5′-CCWGG sequence recognition by the N- and C-terminal domains of EcoRII
por: Golovenko, Dmitrij, et al.
Publicado: (2009)

Structure-guided sequence specificity engineering of the modification-dependent restriction endonuclease LpnPI
por: Sasnauskas, Giedrius, et al.
Publicado: (2015)

Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family
por: Gasiunas, Giedrius, et al.
Publicado: (2008)

Restriction endonuclease BpuJI specific for the 5′-CCCGT sequence is related to the archaeal Holliday junction resolvase family
por: Sukackaite, Rasa, et al.
Publicado: (2007)

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
por: Sapranauskas, Rimantas, et al.
Publicado: (2011)

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus
por: Karvelis, Tautvydas, et al.
Publicado: (2013)

Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System
por: Wilkinson, Martin, et al.
Publicado: (2019)

The dynamics of the monomeric restriction endonuclease BcnI during its interaction with DNA
por: Kostiuk, Georgij, et al.
Publicado: (2017)

Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes
por: Neely, Robert K., et al.
Publicado: (2009)

Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
por: Karvelis, Tautvydas, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_rn0gpa5ksmuim2mkvcrb99fadd