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Development and application of a high-content virion display human GPCR array
Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabricated a Virion Display (VirD) array containing 315 non-olfacto...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491619/ https://www.ncbi.nlm.nih.gov/pubmed/31040288 http://dx.doi.org/10.1038/s41467-019-09938-9 |
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author | Syu, Guan-Da Wang, Shih-Chin Ma, Guangzhong Liu, Shuang Pearce, Donna Prakash, Atish Henson, Brandon Weng, Lien-Chun Ghosh, Devlina Ramos, Pedro Eichinger, Daniel Pino, Ignacio Dong, Xinzhong Xiao, Jie Wang, Shaopeng Tao, Nongjian Kim, Kwang Sik Desai, Prashant J. Zhu, Heng |
author_facet | Syu, Guan-Da Wang, Shih-Chin Ma, Guangzhong Liu, Shuang Pearce, Donna Prakash, Atish Henson, Brandon Weng, Lien-Chun Ghosh, Devlina Ramos, Pedro Eichinger, Daniel Pino, Ignacio Dong, Xinzhong Xiao, Jie Wang, Shaopeng Tao, Nongjian Kim, Kwang Sik Desai, Prashant J. Zhu, Heng |
author_sort | Syu, Guan-Da |
collection | PubMed |
description | Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabricated a Virion Display (VirD) array containing 315 non-olfactory human GPCRs for functional characterization. Using this array, we found that 10 of 20 anti-GPCR mAbs were ultra-specific. We further demonstrated that those failed in the mAb assays could recognize their canonical ligands, suggesting proper folding. Next, using two peptide ligands on the VirD-GPCR array, we identified expected interactions and novel interactions. Finally, we screened the array with group B Streptococcus, a major cause of neonatal meningitis, and demonstrated that inhibition of a newly identified target, CysLTR1, reduced bacterial penetration both in vitro and in vivo. We believe that the VirD-GPCR array holds great potential for high-throughput screening for small molecule drugs, affinity reagents, and ligand deorphanization. |
format | Online Article Text |
id | pubmed-6491619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-64916192019-05-02 Development and application of a high-content virion display human GPCR array Syu, Guan-Da Wang, Shih-Chin Ma, Guangzhong Liu, Shuang Pearce, Donna Prakash, Atish Henson, Brandon Weng, Lien-Chun Ghosh, Devlina Ramos, Pedro Eichinger, Daniel Pino, Ignacio Dong, Xinzhong Xiao, Jie Wang, Shaopeng Tao, Nongjian Kim, Kwang Sik Desai, Prashant J. Zhu, Heng Nat Commun Article Human G protein-coupled receptors (GPCRs) respond to various ligands and stimuli. However, GPCRs rely on membrane for proper folding, making their biochemical properties difficult to study. By displaying GPCRs in viral envelopes, we fabricated a Virion Display (VirD) array containing 315 non-olfactory human GPCRs for functional characterization. Using this array, we found that 10 of 20 anti-GPCR mAbs were ultra-specific. We further demonstrated that those failed in the mAb assays could recognize their canonical ligands, suggesting proper folding. Next, using two peptide ligands on the VirD-GPCR array, we identified expected interactions and novel interactions. Finally, we screened the array with group B Streptococcus, a major cause of neonatal meningitis, and demonstrated that inhibition of a newly identified target, CysLTR1, reduced bacterial penetration both in vitro and in vivo. We believe that the VirD-GPCR array holds great potential for high-throughput screening for small molecule drugs, affinity reagents, and ligand deorphanization. Nature Publishing Group UK 2019-04-30 /pmc/articles/PMC6491619/ /pubmed/31040288 http://dx.doi.org/10.1038/s41467-019-09938-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Syu, Guan-Da Wang, Shih-Chin Ma, Guangzhong Liu, Shuang Pearce, Donna Prakash, Atish Henson, Brandon Weng, Lien-Chun Ghosh, Devlina Ramos, Pedro Eichinger, Daniel Pino, Ignacio Dong, Xinzhong Xiao, Jie Wang, Shaopeng Tao, Nongjian Kim, Kwang Sik Desai, Prashant J. Zhu, Heng Development and application of a high-content virion display human GPCR array |
title | Development and application of a high-content virion display human GPCR array |
title_full | Development and application of a high-content virion display human GPCR array |
title_fullStr | Development and application of a high-content virion display human GPCR array |
title_full_unstemmed | Development and application of a high-content virion display human GPCR array |
title_short | Development and application of a high-content virion display human GPCR array |
title_sort | development and application of a high-content virion display human gpcr array |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491619/ https://www.ncbi.nlm.nih.gov/pubmed/31040288 http://dx.doi.org/10.1038/s41467-019-09938-9 |
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