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Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach
Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491765/ https://www.ncbi.nlm.nih.gov/pubmed/31068959 http://dx.doi.org/10.3389/fpls.2019.00509 |
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author | Amaral, Joana Correia, Barbara António, Carla Rodrigues, Ana Margarida Gómez-Cadenas, Aurelio Valledor, Luis Hancock, Robert D. Alves, Artur Pinto, Glória |
author_facet | Amaral, Joana Correia, Barbara António, Carla Rodrigues, Ana Margarida Gómez-Cadenas, Aurelio Valledor, Luis Hancock, Robert D. Alves, Artur Pinto, Glória |
author_sort | Amaral, Joana |
collection | PubMed |
description | Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus–Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation. |
format | Online Article Text |
id | pubmed-6491765 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64917652019-05-08 Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach Amaral, Joana Correia, Barbara António, Carla Rodrigues, Ana Margarida Gómez-Cadenas, Aurelio Valledor, Luis Hancock, Robert D. Alves, Artur Pinto, Glória Front Plant Sci Plant Science Fusarium circinatum, the causal agent of pine pitch canker (PPC), is an emergent and still understudied risk that threatens Pinus forests worldwide, with potential production and sustainability losses. In order to explore the response of pine species with distinct levels of susceptibility to PPC, we investigated changes in physiology, hormones, specific gene transcripts, and primary metabolism occurring in symptomatic Pinus pinea, Pinus pinaster, and Pinus radiata upon inoculation with F. circinatum. Pinus radiata and P. pinaster exhibiting high and intermediate susceptibility to PPC, respectively, suffered changes in plant water status and photosynthetic impairment. This was associated with sink metabolism induction, a general accumulation of amino acids and overexpression of pathogenesis-related genes. On the other hand, P. pinea exhibited the greatest resistance to PPC and stomatal opening, transpiration increase, and glycerol accumulation were observed in inoculated plants. A stronger induction of pyruvate decarboxylase transcripts and differential hormones regulation were also found for inoculated P. pinea in comparison with the susceptible Pinus species studied. The specific physiological changes reported herein are the first steps to understand the complex Pinus–Fusarium interaction and create tools for the selection of resistant genotypes thus contributing to disease mitigation. Frontiers Media S.A. 2019-04-24 /pmc/articles/PMC6491765/ /pubmed/31068959 http://dx.doi.org/10.3389/fpls.2019.00509 Text en Copyright © 2019 Amaral, Correia, António, Rodrigues, Gómez-Cadenas, Valledor, Hancock, Alves and Pinto. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Amaral, Joana Correia, Barbara António, Carla Rodrigues, Ana Margarida Gómez-Cadenas, Aurelio Valledor, Luis Hancock, Robert D. Alves, Artur Pinto, Glória Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title | Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title_full | Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title_fullStr | Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title_full_unstemmed | Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title_short | Pinus Susceptibility to Pitch Canker Triggers Specific Physiological Responses in Symptomatic Plants: An Integrated Approach |
title_sort | pinus susceptibility to pitch canker triggers specific physiological responses in symptomatic plants: an integrated approach |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491765/ https://www.ncbi.nlm.nih.gov/pubmed/31068959 http://dx.doi.org/10.3389/fpls.2019.00509 |
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