Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates

A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabl...

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Detalles Bibliográficos
Autores principales: Gardner, Andrew F., Jackson, Kiserian M., Boyle, Madeleine M., Buss, Jackson A., Potapov, Vladimir, Gehring, Alexandra M., Zatopek, Kelly M., Corrêa Jr., Ivan R., Ong, Jennifer L., Jack, William E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491775/
https://www.ncbi.nlm.nih.gov/pubmed/31069234
http://dx.doi.org/10.3389/fmolb.2019.00028
Descripción
Sumario:A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.

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