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Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates
A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabl...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491775/ https://www.ncbi.nlm.nih.gov/pubmed/31069234 http://dx.doi.org/10.3389/fmolb.2019.00028 |
| _version_ | 1783415012530847744 |
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| author | Gardner, Andrew F. Jackson, Kiserian M. Boyle, Madeleine M. Buss, Jackson A. Potapov, Vladimir Gehring, Alexandra M. Zatopek, Kelly M. Corrêa Jr., Ivan R. Ong, Jennifer L. Jack, William E. |
| author_facet | Gardner, Andrew F. Jackson, Kiserian M. Boyle, Madeleine M. Buss, Jackson A. Potapov, Vladimir Gehring, Alexandra M. Zatopek, Kelly M. Corrêa Jr., Ivan R. Ong, Jennifer L. Jack, William E. |
| author_sort | Gardner, Andrew F. |
| collection | PubMed |
| description | A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation. |
| format | Online Article Text |
| id | pubmed-6491775 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2019 |
| publisher | Frontiers Media S.A. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-64917752019-05-08 Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates Gardner, Andrew F. Jackson, Kiserian M. Boyle, Madeleine M. Buss, Jackson A. Potapov, Vladimir Gehring, Alexandra M. Zatopek, Kelly M. Corrêa Jr., Ivan R. Ong, Jennifer L. Jack, William E. Front Mol Biosci Molecular Biosciences A variant of 9°N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name “Therminator DNA polymerase” has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation. Frontiers Media S.A. 2019-04-24 /pmc/articles/PMC6491775/ /pubmed/31069234 http://dx.doi.org/10.3389/fmolb.2019.00028 Text en Copyright © 2019 Gardner, Jackson, Boyle, Buss, Potapov, Gehring, Zatopek, Corrêa, Ong and Jack. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
| spellingShingle | Molecular Biosciences Gardner, Andrew F. Jackson, Kiserian M. Boyle, Madeleine M. Buss, Jackson A. Potapov, Vladimir Gehring, Alexandra M. Zatopek, Kelly M. Corrêa Jr., Ivan R. Ong, Jennifer L. Jack, William E. Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title | Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title_full | Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title_fullStr | Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title_full_unstemmed | Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title_short | Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates |
| title_sort | therminator dna polymerase: modified nucleotides and unnatural substrates |
| topic | Molecular Biosciences |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491775/ https://www.ncbi.nlm.nih.gov/pubmed/31069234 http://dx.doi.org/10.3389/fmolb.2019.00028 |
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