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Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells
The purpose was to evaluate the effect of platelets on functional properties of late endothelial progenitor cells (EPCs), in the direct co-culture conditions, and to investigate the involved mediators, in experimental induced atherosclerosis. The late EPCs obtained from two animal groups, hypertensi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491786/ https://www.ncbi.nlm.nih.gov/pubmed/31068820 http://dx.doi.org/10.3389/fphar.2019.00424 |
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author | Alexandru, Nicoleta Safciuc, Florentina Constantin, Alina Nemecz, Miruna Tanko, Gabriela Filippi, Alexandru Dragan, Emanuel Bãdilã, Elisabeta Georgescu, Adriana |
author_facet | Alexandru, Nicoleta Safciuc, Florentina Constantin, Alina Nemecz, Miruna Tanko, Gabriela Filippi, Alexandru Dragan, Emanuel Bãdilã, Elisabeta Georgescu, Adriana |
author_sort | Alexandru, Nicoleta |
collection | PubMed |
description | The purpose was to evaluate the effect of platelets on functional properties of late endothelial progenitor cells (EPCs), in the direct co-culture conditions, and to investigate the involved mediators, in experimental induced atherosclerosis. The late EPCs obtained from two animal groups, hypertensive-hyperlipidemic (HH) and control (C) hamsters, named late EPCs-HH and late EPCs-C, were co-incubated with or without platelets isolated from both groups. Our results have showed that exposure to platelets from control animals: (i) promoted the late EPCs-C capacity to form colonies and capillary-like structures, and also to proliferate and migrate; (ii) improved the functional properties of late EPCs-HH; (iii) strengthened the direct binding EPCs-platelets; (iv) increased SDF-1α,VEGF, PDGF, and reduced CD40L, IL-1β,-6,-8 levels; and (v) enhanced miR-223 and IGF-1R expressions. Platelets from HH group diminished functional abilities for both EPC types and had opposite effects on these pro-angiogenic and pro-inflammatory molecules. Furthermore, testing the direct effect of miR-223 and IGF-1R on late EPCs disclosed that these molecular factors improve late EPC functional properties in atherosclerosis in terms of stimulation of the proliferation and migration abilities. In conclusion, in vitro exposure to platelets of healthy origins had a positive effect on functional properties of atherosclerotic late EPCs. The most likely candidates mediating EPC-platelet interaction can be SDF-1α, VEGF, CD40L, PDGF, IL-1β,-6,-8, miR-223, and IGF-1R. The current study brings evidences that the presence of healthy origin platelets is of utmost importance on functional improvement of EPCs in atherosclerosis. |
format | Online Article Text |
id | pubmed-6491786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64917862019-05-08 Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells Alexandru, Nicoleta Safciuc, Florentina Constantin, Alina Nemecz, Miruna Tanko, Gabriela Filippi, Alexandru Dragan, Emanuel Bãdilã, Elisabeta Georgescu, Adriana Front Pharmacol Pharmacology The purpose was to evaluate the effect of platelets on functional properties of late endothelial progenitor cells (EPCs), in the direct co-culture conditions, and to investigate the involved mediators, in experimental induced atherosclerosis. The late EPCs obtained from two animal groups, hypertensive-hyperlipidemic (HH) and control (C) hamsters, named late EPCs-HH and late EPCs-C, were co-incubated with or without platelets isolated from both groups. Our results have showed that exposure to platelets from control animals: (i) promoted the late EPCs-C capacity to form colonies and capillary-like structures, and also to proliferate and migrate; (ii) improved the functional properties of late EPCs-HH; (iii) strengthened the direct binding EPCs-platelets; (iv) increased SDF-1α,VEGF, PDGF, and reduced CD40L, IL-1β,-6,-8 levels; and (v) enhanced miR-223 and IGF-1R expressions. Platelets from HH group diminished functional abilities for both EPC types and had opposite effects on these pro-angiogenic and pro-inflammatory molecules. Furthermore, testing the direct effect of miR-223 and IGF-1R on late EPCs disclosed that these molecular factors improve late EPC functional properties in atherosclerosis in terms of stimulation of the proliferation and migration abilities. In conclusion, in vitro exposure to platelets of healthy origins had a positive effect on functional properties of atherosclerotic late EPCs. The most likely candidates mediating EPC-platelet interaction can be SDF-1α, VEGF, CD40L, PDGF, IL-1β,-6,-8, miR-223, and IGF-1R. The current study brings evidences that the presence of healthy origin platelets is of utmost importance on functional improvement of EPCs in atherosclerosis. Frontiers Media S.A. 2019-04-24 /pmc/articles/PMC6491786/ /pubmed/31068820 http://dx.doi.org/10.3389/fphar.2019.00424 Text en Copyright © 2019 Alexandru, Safciuc, Constantin, Nemecz, Tanko, Filippi, Dragan, Bãdilã and Georgescu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Alexandru, Nicoleta Safciuc, Florentina Constantin, Alina Nemecz, Miruna Tanko, Gabriela Filippi, Alexandru Dragan, Emanuel Bãdilã, Elisabeta Georgescu, Adriana Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title | Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title_full | Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title_fullStr | Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title_full_unstemmed | Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title_short | Platelets of Healthy Origins Promote Functional Improvement of Atherosclerotic Endothelial Progenitor Cells |
title_sort | platelets of healthy origins promote functional improvement of atherosclerotic endothelial progenitor cells |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491786/ https://www.ncbi.nlm.nih.gov/pubmed/31068820 http://dx.doi.org/10.3389/fphar.2019.00424 |
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