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Disaccharides obtained from carrageenans as potential antitumor agents

Carrageenans are sulfated galactans found in certain red seaweeds with proven biological activities. In this work, we have prepared purified native and degraded κ-, ι-; and λ-carrageenans, including the disaccharides (carrabioses) and disaccharide-alditols (carrabiitols) from seaweed extracts as pot...

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Autores principales: Calvo, Gustavo H., Cosenza, Vanina A., Sáenz, Daniel A., Navarro, Diego A., Stortz, Carlos A., Céspedes, Mariela A., Mamone, Leandro A., Casas, Adriana G., Di Venosa, Gabriela M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491800/
https://www.ncbi.nlm.nih.gov/pubmed/31040376
http://dx.doi.org/10.1038/s41598-019-43238-y
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author Calvo, Gustavo H.
Cosenza, Vanina A.
Sáenz, Daniel A.
Navarro, Diego A.
Stortz, Carlos A.
Céspedes, Mariela A.
Mamone, Leandro A.
Casas, Adriana G.
Di Venosa, Gabriela M.
author_facet Calvo, Gustavo H.
Cosenza, Vanina A.
Sáenz, Daniel A.
Navarro, Diego A.
Stortz, Carlos A.
Céspedes, Mariela A.
Mamone, Leandro A.
Casas, Adriana G.
Di Venosa, Gabriela M.
author_sort Calvo, Gustavo H.
collection PubMed
description Carrageenans are sulfated galactans found in certain red seaweeds with proven biological activities. In this work, we have prepared purified native and degraded κ-, ι-; and λ-carrageenans, including the disaccharides (carrabioses) and disaccharide-alditols (carrabiitols) from seaweed extracts as potential antitumor compounds and identified the active principle of the cytotoxic and potential antitumor properties of these compounds. Both κ and ι-carrageenan, as well as carrageenan oligosaccharides showed cytotoxic effect over LM2 tumor cells. Characterized disaccharides (carrabioses) and the reduced product carrabiitols, were also tested. Only carrabioses were cytotoxic, and among them, κ-carrabiose was the most effective, showing high cytotoxic properties, killing the cells through an apoptotic pathway. In addition, the cells surviving treatment with κ-carrabiose, showed a decreased metastatic ability in vitro, together with a decreased cell-cell and cell-matrix interactions, thus suggesting possible antitumor potential. Overall, our results indicate that most cytotoxic compounds derived from carrageenans have lower molecular weights and sulfate content. Potential applications of the results emerging from the present work include the use of disaccharide units such as carrabioses coupled to antineoplasics in order to improve its cytotoxicity and antimetastatic properties, and the use of ι-carrageenan as adjuvant or carrier in anticancer treatments.
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spelling pubmed-64918002019-05-17 Disaccharides obtained from carrageenans as potential antitumor agents Calvo, Gustavo H. Cosenza, Vanina A. Sáenz, Daniel A. Navarro, Diego A. Stortz, Carlos A. Céspedes, Mariela A. Mamone, Leandro A. Casas, Adriana G. Di Venosa, Gabriela M. Sci Rep Article Carrageenans are sulfated galactans found in certain red seaweeds with proven biological activities. In this work, we have prepared purified native and degraded κ-, ι-; and λ-carrageenans, including the disaccharides (carrabioses) and disaccharide-alditols (carrabiitols) from seaweed extracts as potential antitumor compounds and identified the active principle of the cytotoxic and potential antitumor properties of these compounds. Both κ and ι-carrageenan, as well as carrageenan oligosaccharides showed cytotoxic effect over LM2 tumor cells. Characterized disaccharides (carrabioses) and the reduced product carrabiitols, were also tested. Only carrabioses were cytotoxic, and among them, κ-carrabiose was the most effective, showing high cytotoxic properties, killing the cells through an apoptotic pathway. In addition, the cells surviving treatment with κ-carrabiose, showed a decreased metastatic ability in vitro, together with a decreased cell-cell and cell-matrix interactions, thus suggesting possible antitumor potential. Overall, our results indicate that most cytotoxic compounds derived from carrageenans have lower molecular weights and sulfate content. Potential applications of the results emerging from the present work include the use of disaccharide units such as carrabioses coupled to antineoplasics in order to improve its cytotoxicity and antimetastatic properties, and the use of ι-carrageenan as adjuvant or carrier in anticancer treatments. Nature Publishing Group UK 2019-04-30 /pmc/articles/PMC6491800/ /pubmed/31040376 http://dx.doi.org/10.1038/s41598-019-43238-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Calvo, Gustavo H.
Cosenza, Vanina A.
Sáenz, Daniel A.
Navarro, Diego A.
Stortz, Carlos A.
Céspedes, Mariela A.
Mamone, Leandro A.
Casas, Adriana G.
Di Venosa, Gabriela M.
Disaccharides obtained from carrageenans as potential antitumor agents
title Disaccharides obtained from carrageenans as potential antitumor agents
title_full Disaccharides obtained from carrageenans as potential antitumor agents
title_fullStr Disaccharides obtained from carrageenans as potential antitumor agents
title_full_unstemmed Disaccharides obtained from carrageenans as potential antitumor agents
title_short Disaccharides obtained from carrageenans as potential antitumor agents
title_sort disaccharides obtained from carrageenans as potential antitumor agents
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491800/
https://www.ncbi.nlm.nih.gov/pubmed/31040376
http://dx.doi.org/10.1038/s41598-019-43238-y
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