An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species

Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides prot...

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Autores principales: Chibssa, Tesfaye Rufael, Settypalli, Tirumala Bharani K., Berguido, Francisco J., Grabherr, Reingard, Loitsch, Angelika, Tuppurainen, Eeva, Nwankpa, Nick, Tounkara, Karim, Madani, Hafsa, Omani, Amel, Diop, Mariane, Cattoli, Giovanni, Diallo, Adama, Lamien, Charles Euloge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491823/
https://www.ncbi.nlm.nih.gov/pubmed/31040355
http://dx.doi.org/10.1038/s41598-019-43158-x
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author Chibssa, Tesfaye Rufael
Settypalli, Tirumala Bharani K.
Berguido, Francisco J.
Grabherr, Reingard
Loitsch, Angelika
Tuppurainen, Eeva
Nwankpa, Nick
Tounkara, Karim
Madani, Hafsa
Omani, Amel
Diop, Mariane
Cattoli, Giovanni
Diallo, Adama
Lamien, Charles Euloge
author_facet Chibssa, Tesfaye Rufael
Settypalli, Tirumala Bharani K.
Berguido, Francisco J.
Grabherr, Reingard
Loitsch, Angelika
Tuppurainen, Eeva
Nwankpa, Nick
Tounkara, Karim
Madani, Hafsa
Omani, Amel
Diop, Mariane
Cattoli, Giovanni
Diallo, Adama
Lamien, Charles Euloge
author_sort Chibssa, Tesfaye Rufael
collection PubMed
description Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates.
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spelling pubmed-64918232019-05-17 An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species Chibssa, Tesfaye Rufael Settypalli, Tirumala Bharani K. Berguido, Francisco J. Grabherr, Reingard Loitsch, Angelika Tuppurainen, Eeva Nwankpa, Nick Tounkara, Karim Madani, Hafsa Omani, Amel Diop, Mariane Cattoli, Giovanni Diallo, Adama Lamien, Charles Euloge Sci Rep Article Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates. Nature Publishing Group UK 2019-04-30 /pmc/articles/PMC6491823/ /pubmed/31040355 http://dx.doi.org/10.1038/s41598-019-43158-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Chibssa, Tesfaye Rufael
Settypalli, Tirumala Bharani K.
Berguido, Francisco J.
Grabherr, Reingard
Loitsch, Angelika
Tuppurainen, Eeva
Nwankpa, Nick
Tounkara, Karim
Madani, Hafsa
Omani, Amel
Diop, Mariane
Cattoli, Giovanni
Diallo, Adama
Lamien, Charles Euloge
An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title_full An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title_fullStr An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title_full_unstemmed An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title_short An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species
title_sort hrm assay to differentiate sheeppox virus vaccine strains from sheeppox virus field isolates and other capripoxvirus species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491823/
https://www.ncbi.nlm.nih.gov/pubmed/31040355
http://dx.doi.org/10.1038/s41598-019-43158-x
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