A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo

The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with dif...

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Autores principales: Dalal, Jasmer, Kumar, Pradeep, Chandolia, R. K., Pawaria, Shikha, Rajendran, Rasika, Sheoran, Suman, Andonissamy, Jerome, Kumar, Dharmendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491824/
https://www.ncbi.nlm.nih.gov/pubmed/31040304
http://dx.doi.org/10.1038/s41598-019-43038-4
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author Dalal, Jasmer
Kumar, Pradeep
Chandolia, R. K.
Pawaria, Shikha
Rajendran, Rasika
Sheoran, Suman
Andonissamy, Jerome
Kumar, Dharmendra
author_facet Dalal, Jasmer
Kumar, Pradeep
Chandolia, R. K.
Pawaria, Shikha
Rajendran, Rasika
Sheoran, Suman
Andonissamy, Jerome
Kumar, Dharmendra
author_sort Dalal, Jasmer
collection PubMed
description The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations of RU 486 (0, 5, 10 and 20 µM) and cryopreserved. We found that RU 486 did not impair the post-thaw sperm kinetics and motility but prevented cholesterol efflux, calcium influx, and protected CatSper channels during cryopreservation. The RU 486 protected sperm from premature capacitation which was confirmed by intracellular calcium level, expression of tyrosine phosphorylated proteins (75 and 80 kDa) and CTC (chlortetracycline) assay. Furthermore, antioxidant ability of RU 486 was reflected by the ferric reducing ability, lower production of sperm malondialdehyde and intracellular reactive oxygen species. Also, we demonstrated that RU 486 treated sperm underwent normal capacitation, zona pellucida binding and zygote cleavage indicating normal fertilizing ability of sperm. In conclusion, we report a new role of RU 486 in protecting buffalo sperm from premature capacitation during cryopreservation.
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spelling pubmed-64918242019-05-17 A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo Dalal, Jasmer Kumar, Pradeep Chandolia, R. K. Pawaria, Shikha Rajendran, Rasika Sheoran, Suman Andonissamy, Jerome Kumar, Dharmendra Sci Rep Article The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations of RU 486 (0, 5, 10 and 20 µM) and cryopreserved. We found that RU 486 did not impair the post-thaw sperm kinetics and motility but prevented cholesterol efflux, calcium influx, and protected CatSper channels during cryopreservation. The RU 486 protected sperm from premature capacitation which was confirmed by intracellular calcium level, expression of tyrosine phosphorylated proteins (75 and 80 kDa) and CTC (chlortetracycline) assay. Furthermore, antioxidant ability of RU 486 was reflected by the ferric reducing ability, lower production of sperm malondialdehyde and intracellular reactive oxygen species. Also, we demonstrated that RU 486 treated sperm underwent normal capacitation, zona pellucida binding and zygote cleavage indicating normal fertilizing ability of sperm. In conclusion, we report a new role of RU 486 in protecting buffalo sperm from premature capacitation during cryopreservation. Nature Publishing Group UK 2019-04-30 /pmc/articles/PMC6491824/ /pubmed/31040304 http://dx.doi.org/10.1038/s41598-019-43038-4 Text en © The Author(s) 2019, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Dalal, Jasmer
Kumar, Pradeep
Chandolia, R. K.
Pawaria, Shikha
Rajendran, Rasika
Sheoran, Suman
Andonissamy, Jerome
Kumar, Dharmendra
A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title_full A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title_fullStr A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title_full_unstemmed A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title_short A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
title_sort new role for ru486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491824/
https://www.ncbi.nlm.nih.gov/pubmed/31040304
http://dx.doi.org/10.1038/s41598-019-43038-4
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