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First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Enterocytozoon bieneusi is a common intestinal pathogen in a variety of animals. While E. bieneusi genotypes have become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the T...

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Autores principales: Luo, Run, Xiang, Leiqiong, Liu, Haifeng, Zhong, Zhijun, Liu, Li, Deng, Lei, Liu, Ling, Huang, Xiangming, Zhou, Ziyao, Fu, Hualin, Luo, Yan, Peng, Guangneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492536/
https://www.ncbi.nlm.nih.gov/pubmed/31041895
http://dx.doi.org/10.1051/parasite/2019021
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author Luo, Run
Xiang, Leiqiong
Liu, Haifeng
Zhong, Zhijun
Liu, Li
Deng, Lei
Liu, Ling
Huang, Xiangming
Zhou, Ziyao
Fu, Hualin
Luo, Yan
Peng, Guangneng
author_facet Luo, Run
Xiang, Leiqiong
Liu, Haifeng
Zhong, Zhijun
Liu, Li
Deng, Lei
Liu, Ling
Huang, Xiangming
Zhou, Ziyao
Fu, Hualin
Luo, Yan
Peng, Guangneng
author_sort Luo, Run
collection PubMed
description Enterocytozoon bieneusi is a common intestinal pathogen in a variety of animals. While E. bieneusi genotypes have become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. Enterocytozoon bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan, and 26.7% in Qionglai. Prevalence varies according to age group, from 24.4% (age 0–1 years) to 44.4% (age 1–2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n = 58), Henan-IV (n = 24) and two novel genotypes, SCT01 and SCT02 (one of each). We compare our results with a compilation of published results on the host range and geographical distribution of E. bieneusi genotypes in China. Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis of three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) was successful in 47, 48, 23 and 47 positive specimens and identified 10, 10, 5 and 5 genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic advice for preventing and controlling infections.
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spelling pubmed-64925362019-05-03 First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China Luo, Run Xiang, Leiqiong Liu, Haifeng Zhong, Zhijun Liu, Li Deng, Lei Liu, Ling Huang, Xiangming Zhou, Ziyao Fu, Hualin Luo, Yan Peng, Guangneng Parasite Research Article Enterocytozoon bieneusi is a common intestinal pathogen in a variety of animals. While E. bieneusi genotypes have become better-known, there are few reports on its prevalence in the Tibetan pig. This study investigated the prevalence, genetic diversity, and zoonotic potential of E. bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) were collected from three sites in the southwest of China. Feces were subjected to PCR amplification of the internal transcribed spacer (ITS) region. Enterocytozoon bieneusi was detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in Kangding, 56% in Yaan, and 26.7% in Qionglai. Prevalence varies according to age group, from 24.4% (age 0–1 years) to 44.4% (age 1–2 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC (n = 58), Henan-IV (n = 24) and two novel genotypes, SCT01 and SCT02 (one of each). We compare our results with a compilation of published results on the host range and geographical distribution of E. bieneusi genotypes in China. Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic potential. Multilocus sequence typing (MLST) analysis of three microsatellites (MS1, MS3, MS7) and one minisatellite (MS4) was successful in 47, 48, 23 and 47 positive specimens and identified 10, 10, 5 and 5 genotypes at four loci, respectively. This study indicates the potential danger of E. bieneusi to Tibetan pigs in southwestern China, and offers basic advice for preventing and controlling infections. EDP Sciences 2019-05-01 /pmc/articles/PMC6492536/ /pubmed/31041895 http://dx.doi.org/10.1051/parasite/2019021 Text en © R. Luo et al., published by EDP Sciences, 2019 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Luo, Run
Xiang, Leiqiong
Liu, Haifeng
Zhong, Zhijun
Liu, Li
Deng, Lei
Liu, Ling
Huang, Xiangming
Zhou, Ziyao
Fu, Hualin
Luo, Yan
Peng, Guangneng
First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title_full First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title_fullStr First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title_full_unstemmed First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title_short First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China
title_sort first report and multilocus genotyping of enterocytozoon bieneusi from tibetan pigs in southwestern china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492536/
https://www.ncbi.nlm.nih.gov/pubmed/31041895
http://dx.doi.org/10.1051/parasite/2019021
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