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The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma

The role of tumor-associated macrophages (TAMs) in cutaneous melanoma is controversial. TAMs include immunogenic and immunosuppressive subtypes, and have distinct functions according to their microanatomical localization. Our aim was to investigate TAMs in benign, premalignant, and malignant melanoc...

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Detalles Bibliográficos
Autores principales: Salmi, Satu, Siiskonen, Hanna, Sironen, Reijo, Tyynelä-Korhonen, Kristiina, Hirschovits-Gerz, Benjamin, Valkonen, Mari, Auvinen, Päivi, Pasonen-Seppänen, Sanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Lippincott Williams & Wilkins 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6493694/
https://www.ncbi.nlm.nih.gov/pubmed/30399061
http://dx.doi.org/10.1097/CMR.0000000000000522
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author Salmi, Satu
Siiskonen, Hanna
Sironen, Reijo
Tyynelä-Korhonen, Kristiina
Hirschovits-Gerz, Benjamin
Valkonen, Mari
Auvinen, Päivi
Pasonen-Seppänen, Sanna
author_facet Salmi, Satu
Siiskonen, Hanna
Sironen, Reijo
Tyynelä-Korhonen, Kristiina
Hirschovits-Gerz, Benjamin
Valkonen, Mari
Auvinen, Päivi
Pasonen-Seppänen, Sanna
author_sort Salmi, Satu
collection PubMed
description The role of tumor-associated macrophages (TAMs) in cutaneous melanoma is controversial. TAMs include immunogenic and immunosuppressive subtypes, and have distinct functions according to their microanatomical localization. Our aim was to investigate TAMs in benign, premalignant, and malignant melanocytic lesions to determine possible associations with tumor progression and clinicopathological characteristics. In total, 184 tissue samples, including benign and dysplastic nevi, in-situ melanomas, superficial (Breslow’s depth <1 mm), and deep (Breslow’s depth >4 mm) invasive melanomas and lymph node metastases, were analyzed for macrophage content. Samples were stained immunohistochemically for CD68 and CD163, representing all TAMs and M2-macrophages, respectively. Macrophages were counted by hotspot analysis, and assessed semiquantitatively from the tumor cell nests and stromal component of malignant cases. CD68+ and CD163+ TAMs were more abundant in invasive melanomas compared with benign nevi. The proportion of TAMs in the tumor nests was higher in deep melanomas and lymph node metastases compared with superficially invasive melanomas. High amounts of CD68+ macrophages in tumor cell nests were associated with recurrence, whereas low CD163+ macrophage proportion in tumor stroma was associated with recurrence and in primary melanomas also with poor overall survival. TAMs seem to promote tumor progression in cutaneous melanoma. In particular, CD68+ TAMs and their abundance in tumor nests were associated with poor prognostic factors. However, the correlation of low stromal CD163+ TAM proportion with a poor prognosis indicates that the role of TAMs depends on their subtype and microanatomical localization.
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spelling pubmed-64936942019-05-29 The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma Salmi, Satu Siiskonen, Hanna Sironen, Reijo Tyynelä-Korhonen, Kristiina Hirschovits-Gerz, Benjamin Valkonen, Mari Auvinen, Päivi Pasonen-Seppänen, Sanna Melanoma Res ORIGINAL ARTICLES: Basic science The role of tumor-associated macrophages (TAMs) in cutaneous melanoma is controversial. TAMs include immunogenic and immunosuppressive subtypes, and have distinct functions according to their microanatomical localization. Our aim was to investigate TAMs in benign, premalignant, and malignant melanocytic lesions to determine possible associations with tumor progression and clinicopathological characteristics. In total, 184 tissue samples, including benign and dysplastic nevi, in-situ melanomas, superficial (Breslow’s depth <1 mm), and deep (Breslow’s depth >4 mm) invasive melanomas and lymph node metastases, were analyzed for macrophage content. Samples were stained immunohistochemically for CD68 and CD163, representing all TAMs and M2-macrophages, respectively. Macrophages were counted by hotspot analysis, and assessed semiquantitatively from the tumor cell nests and stromal component of malignant cases. CD68+ and CD163+ TAMs were more abundant in invasive melanomas compared with benign nevi. The proportion of TAMs in the tumor nests was higher in deep melanomas and lymph node metastases compared with superficially invasive melanomas. High amounts of CD68+ macrophages in tumor cell nests were associated with recurrence, whereas low CD163+ macrophage proportion in tumor stroma was associated with recurrence and in primary melanomas also with poor overall survival. TAMs seem to promote tumor progression in cutaneous melanoma. In particular, CD68+ TAMs and their abundance in tumor nests were associated with poor prognostic factors. However, the correlation of low stromal CD163+ TAM proportion with a poor prognosis indicates that the role of TAMs depends on their subtype and microanatomical localization. Lippincott Williams & Wilkins 2019-06 2018-11-05 /pmc/articles/PMC6493694/ /pubmed/30399061 http://dx.doi.org/10.1097/CMR.0000000000000522 Text en Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (http://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle ORIGINAL ARTICLES: Basic science
Salmi, Satu
Siiskonen, Hanna
Sironen, Reijo
Tyynelä-Korhonen, Kristiina
Hirschovits-Gerz, Benjamin
Valkonen, Mari
Auvinen, Päivi
Pasonen-Seppänen, Sanna
The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title_full The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title_fullStr The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title_full_unstemmed The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title_short The number and localization of CD68+ and CD163+ macrophages in different stages of cutaneous melanoma
title_sort number and localization of cd68+ and cd163+ macrophages in different stages of cutaneous melanoma
topic ORIGINAL ARTICLES: Basic science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6493694/
https://www.ncbi.nlm.nih.gov/pubmed/30399061
http://dx.doi.org/10.1097/CMR.0000000000000522
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