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Comparative analysis of widely used methods to remove nonfunctional myosin heads for the in vitro motility assay
The in vitro motility assay allows studies of muscle contraction through observation of actin filament propulsion by surface-adsorbed myosin motors or motor fragments isolated from muscle. A possible problem is that motility may be compromised by nonfunctional, “dead”, motors, obtained in the isolat...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer International Publishing
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6494787/ https://www.ncbi.nlm.nih.gov/pubmed/30850933 http://dx.doi.org/10.1007/s10974-019-09505-1 |