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Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders

Small intestinal bacterial overgrowth (SIBO) has been implicated in symptoms associated with functional gastrointestinal disorders (FGIDs), though mechanisms remain poorly defined and treatment involves non-specific antibiotics. Here we show that SIBO based on duodenal aspirate culture reflects an o...

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Autores principales: Saffouri, George B., Shields-Cutler, Robin R., Chen, Jun, Yang, Yi, Lekatz, Heather R., Hale, Vanessa L., Cho, Janice M., Battaglioli, Eric J., Bhattarai, Yogesh, Thompson, Kevin J., Kalari, Krishna K., Behera, Gaurav, Berry, Jonathan C., Peters, Stephanie A., Patel, Robin, Schuetz, Audrey N., Faith, Jeremiah J., Camilleri, Michael, Sonnenburg, Justin L., Farrugia, Gianrico, Swann, Jonathan R., Grover, Madhusudan, Knights, Dan, Kashyap, Purna C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6494866/
https://www.ncbi.nlm.nih.gov/pubmed/31043597
http://dx.doi.org/10.1038/s41467-019-09964-7
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author Saffouri, George B.
Shields-Cutler, Robin R.
Chen, Jun
Yang, Yi
Lekatz, Heather R.
Hale, Vanessa L.
Cho, Janice M.
Battaglioli, Eric J.
Bhattarai, Yogesh
Thompson, Kevin J.
Kalari, Krishna K.
Behera, Gaurav
Berry, Jonathan C.
Peters, Stephanie A.
Patel, Robin
Schuetz, Audrey N.
Faith, Jeremiah J.
Camilleri, Michael
Sonnenburg, Justin L.
Farrugia, Gianrico
Swann, Jonathan R.
Grover, Madhusudan
Knights, Dan
Kashyap, Purna C.
author_facet Saffouri, George B.
Shields-Cutler, Robin R.
Chen, Jun
Yang, Yi
Lekatz, Heather R.
Hale, Vanessa L.
Cho, Janice M.
Battaglioli, Eric J.
Bhattarai, Yogesh
Thompson, Kevin J.
Kalari, Krishna K.
Behera, Gaurav
Berry, Jonathan C.
Peters, Stephanie A.
Patel, Robin
Schuetz, Audrey N.
Faith, Jeremiah J.
Camilleri, Michael
Sonnenburg, Justin L.
Farrugia, Gianrico
Swann, Jonathan R.
Grover, Madhusudan
Knights, Dan
Kashyap, Purna C.
author_sort Saffouri, George B.
collection PubMed
description Small intestinal bacterial overgrowth (SIBO) has been implicated in symptoms associated with functional gastrointestinal disorders (FGIDs), though mechanisms remain poorly defined and treatment involves non-specific antibiotics. Here we show that SIBO based on duodenal aspirate culture reflects an overgrowth of anaerobes, does not correspond with patient symptoms, and may be a result of dietary preferences. Small intestinal microbial composition, on the other hand, is significantly altered in symptomatic patients and does not correspond with aspirate culture results. In a pilot interventional study we found that switching from a high fiber diet to a low fiber, high simple sugar diet triggered FGID-related symptoms and decreased small intestinal microbial diversity while increasing small intestinal permeability. Our findings demonstrate that characterizing small intestinal microbiomes in patients with gastrointestinal symptoms may allow a more targeted antibacterial or a diet-based approach to treatment.
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spelling pubmed-64948662019-05-03 Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders Saffouri, George B. Shields-Cutler, Robin R. Chen, Jun Yang, Yi Lekatz, Heather R. Hale, Vanessa L. Cho, Janice M. Battaglioli, Eric J. Bhattarai, Yogesh Thompson, Kevin J. Kalari, Krishna K. Behera, Gaurav Berry, Jonathan C. Peters, Stephanie A. Patel, Robin Schuetz, Audrey N. Faith, Jeremiah J. Camilleri, Michael Sonnenburg, Justin L. Farrugia, Gianrico Swann, Jonathan R. Grover, Madhusudan Knights, Dan Kashyap, Purna C. Nat Commun Article Small intestinal bacterial overgrowth (SIBO) has been implicated in symptoms associated with functional gastrointestinal disorders (FGIDs), though mechanisms remain poorly defined and treatment involves non-specific antibiotics. Here we show that SIBO based on duodenal aspirate culture reflects an overgrowth of anaerobes, does not correspond with patient symptoms, and may be a result of dietary preferences. Small intestinal microbial composition, on the other hand, is significantly altered in symptomatic patients and does not correspond with aspirate culture results. In a pilot interventional study we found that switching from a high fiber diet to a low fiber, high simple sugar diet triggered FGID-related symptoms and decreased small intestinal microbial diversity while increasing small intestinal permeability. Our findings demonstrate that characterizing small intestinal microbiomes in patients with gastrointestinal symptoms may allow a more targeted antibacterial or a diet-based approach to treatment. Nature Publishing Group UK 2019-05-01 /pmc/articles/PMC6494866/ /pubmed/31043597 http://dx.doi.org/10.1038/s41467-019-09964-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Saffouri, George B.
Shields-Cutler, Robin R.
Chen, Jun
Yang, Yi
Lekatz, Heather R.
Hale, Vanessa L.
Cho, Janice M.
Battaglioli, Eric J.
Bhattarai, Yogesh
Thompson, Kevin J.
Kalari, Krishna K.
Behera, Gaurav
Berry, Jonathan C.
Peters, Stephanie A.
Patel, Robin
Schuetz, Audrey N.
Faith, Jeremiah J.
Camilleri, Michael
Sonnenburg, Justin L.
Farrugia, Gianrico
Swann, Jonathan R.
Grover, Madhusudan
Knights, Dan
Kashyap, Purna C.
Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title_full Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title_fullStr Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title_full_unstemmed Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title_short Small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
title_sort small intestinal microbial dysbiosis underlies symptoms associated with functional gastrointestinal disorders
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6494866/
https://www.ncbi.nlm.nih.gov/pubmed/31043597
http://dx.doi.org/10.1038/s41467-019-09964-7
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