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miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer

MicroRNAs have been proposed as novel biomarkers for the diagnosis and treatment of many types of cancer. The levels of five candidate microRNAs (miRNAs) (miR-99a-5p, miR-31-5p, miR-138-5p, miR-21-5p, and miR-375-3p) in sera from oral cancer patients and paired tumor and normal tissues were detected...

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Detalles Bibliográficos
Autores principales: Lu, Zhiyuan, He, Qianting, Liang, Jianfeng, Li, Wuguo, Su, Qiao, Chen, Zujian, Wan, Quan, Zhou, Xiaofeng, Cao, Laurel, Sun, Jingjing, Wu, Yu, Liu, Lin, Wu, Xinming, Hou, Jinsong, Lian, Keqian, Wang, Anxun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6495075/
https://www.ncbi.nlm.nih.gov/pubmed/31051332
http://dx.doi.org/10.1016/j.omtn.2019.03.012
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author Lu, Zhiyuan
He, Qianting
Liang, Jianfeng
Li, Wuguo
Su, Qiao
Chen, Zujian
Wan, Quan
Zhou, Xiaofeng
Cao, Laurel
Sun, Jingjing
Wu, Yu
Liu, Lin
Wu, Xinming
Hou, Jinsong
Lian, Keqian
Wang, Anxun
author_facet Lu, Zhiyuan
He, Qianting
Liang, Jianfeng
Li, Wuguo
Su, Qiao
Chen, Zujian
Wan, Quan
Zhou, Xiaofeng
Cao, Laurel
Sun, Jingjing
Wu, Yu
Liu, Lin
Wu, Xinming
Hou, Jinsong
Lian, Keqian
Wang, Anxun
author_sort Lu, Zhiyuan
collection PubMed
description MicroRNAs have been proposed as novel biomarkers for the diagnosis and treatment of many types of cancer. The levels of five candidate microRNAs (miRNAs) (miR-99a-5p, miR-31-5p, miR-138-5p, miR-21-5p, and miR-375-3p) in sera from oral cancer patients and paired tumor and normal tissues were detected by real-time qPCR. The diagnostic power of these miRNAs was analyzed by receiver operating characteristic (ROC) curves. Patient-derived xenograft (PDX) models of oral cancer were established and utilized to verify the potential therapeutic effect of miR-31-5p. Candidate miRNAs were screened from our previous studies and verified in 11 paired oral cancer and adjacent normal tissues. Only serum miR-31-5p levels were significantly different between oral cancer patients and healthy controls and between pre- and postoperative patients. Based on the logistic regression model, this panel of five miRNAs distinguished oral cancer patients from healthy control, with an area under the ROC curve (AUC) of 0.776 (sensitivity = 76.8% and specificity = 73.6%). Furthermore, a miR-31-5p mimic enhanced the proliferation of normal epithelial cells, and antagomiR-31-5p inhibited the proliferation of oral cancer cells in vitro. In vivo, antagomiR-31-5p significantly inhibited tumor growth in oral cancer PDX models. Our findings suggest that circulating miR-31-5p might act as an independent biomarker for oral cancer diagnosis and could serve as a therapeutic target for oral cancer.
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spelling pubmed-64950752019-05-06 miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer Lu, Zhiyuan He, Qianting Liang, Jianfeng Li, Wuguo Su, Qiao Chen, Zujian Wan, Quan Zhou, Xiaofeng Cao, Laurel Sun, Jingjing Wu, Yu Liu, Lin Wu, Xinming Hou, Jinsong Lian, Keqian Wang, Anxun Mol Ther Nucleic Acids Article MicroRNAs have been proposed as novel biomarkers for the diagnosis and treatment of many types of cancer. The levels of five candidate microRNAs (miRNAs) (miR-99a-5p, miR-31-5p, miR-138-5p, miR-21-5p, and miR-375-3p) in sera from oral cancer patients and paired tumor and normal tissues were detected by real-time qPCR. The diagnostic power of these miRNAs was analyzed by receiver operating characteristic (ROC) curves. Patient-derived xenograft (PDX) models of oral cancer were established and utilized to verify the potential therapeutic effect of miR-31-5p. Candidate miRNAs were screened from our previous studies and verified in 11 paired oral cancer and adjacent normal tissues. Only serum miR-31-5p levels were significantly different between oral cancer patients and healthy controls and between pre- and postoperative patients. Based on the logistic regression model, this panel of five miRNAs distinguished oral cancer patients from healthy control, with an area under the ROC curve (AUC) of 0.776 (sensitivity = 76.8% and specificity = 73.6%). Furthermore, a miR-31-5p mimic enhanced the proliferation of normal epithelial cells, and antagomiR-31-5p inhibited the proliferation of oral cancer cells in vitro. In vivo, antagomiR-31-5p significantly inhibited tumor growth in oral cancer PDX models. Our findings suggest that circulating miR-31-5p might act as an independent biomarker for oral cancer diagnosis and could serve as a therapeutic target for oral cancer. American Society of Gene & Cell Therapy 2019-04-11 /pmc/articles/PMC6495075/ /pubmed/31051332 http://dx.doi.org/10.1016/j.omtn.2019.03.012 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lu, Zhiyuan
He, Qianting
Liang, Jianfeng
Li, Wuguo
Su, Qiao
Chen, Zujian
Wan, Quan
Zhou, Xiaofeng
Cao, Laurel
Sun, Jingjing
Wu, Yu
Liu, Lin
Wu, Xinming
Hou, Jinsong
Lian, Keqian
Wang, Anxun
miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title_full miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title_fullStr miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title_full_unstemmed miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title_short miR-31-5p Is a Potential Circulating Biomarker and Therapeutic Target for Oral Cancer
title_sort mir-31-5p is a potential circulating biomarker and therapeutic target for oral cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6495075/
https://www.ncbi.nlm.nih.gov/pubmed/31051332
http://dx.doi.org/10.1016/j.omtn.2019.03.012
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