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IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation
Background: Growing evidence has indicated that interleukin-37 (IL-37) is a potential anticancer molecule that mainly plays an inhibiting role in different kinds of cancers, but data for the role of IL-37 on cell apoptosis in cancers remains rare. The present study aimed to explore the role of IL-37...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497894/ https://www.ncbi.nlm.nih.gov/pubmed/31114224 http://dx.doi.org/10.2147/OTT.S201664 |
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author | Ouyang, Ping An, Weifang Chen, Renhuai Zhang, He Chen, Danrui Jiang, Enping Zhu, Wei Li, Peng Guo, Hongsheng Chen, Zhangquan Wang, Sen |
author_facet | Ouyang, Ping An, Weifang Chen, Renhuai Zhang, He Chen, Danrui Jiang, Enping Zhu, Wei Li, Peng Guo, Hongsheng Chen, Zhangquan Wang, Sen |
author_sort | Ouyang, Ping |
collection | PubMed |
description | Background: Growing evidence has indicated that interleukin-37 (IL-37) is a potential anticancer molecule that mainly plays an inhibiting role in different kinds of cancers, but data for the role of IL-37 on cell apoptosis in cancers remains rare. The present study aimed to explore the role of IL-37 in cell apoptosis in cervical cancer, and the involved apoptosis-related molecules. Methods: IL-37 was overexpressed by transfecting the pIRES2-EGFP-IL-37 plasmid in HeLa and C33A cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect the mRNA expression of IL-37, Bcl-2, Bax and Bim. Western blotting was performed to detect the protein expression of IL-37 and Bim. Cell apoptosis was detected by flow cytometry. Results: IL-37 upregulated the mRNA expression levels of Bim by 138.40% for HeLa (P<0.05) and 58.95% for C33A (P<0.05), and increased the protein expression levels of BimL by 69.10% (P<0.05) and 56.66% (P<0.05) in HeLa and C33A, respectively. Overexpression of IL-37 increased the apoptosis rates by 152.86% for HeLa (P<0.01) and 25.4% for C33A (P<0.05). Knockdown of Bim by specific siRNA interference fragments (SiBim) reduced the apoptosis rates by 36.00% for HeLa (P<0.05) and 14.66% for C33A (P<0.05). Compared with the IL-37 overexpression group, the apoptosis rate in cotransfecting the IL-37 overexpression plasmid and SiBim group decreased by approximately 31% (P<0.05) and 24.35% (P<0.05) in HeLa and C33A, respectively. Conclusion: IL-37 upregulated Bim in cervical cancer cells. Furthermore, IL-37 can promote cervical cancer cell apoptosis, but Bim knockdown decreased this promotion through IL-37. Thus, IL-37 can promote cervical cancer cell apoptosis, which involve the upregulation of Bim. |
format | Online Article Text |
id | pubmed-6497894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-64978942019-05-21 IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation Ouyang, Ping An, Weifang Chen, Renhuai Zhang, He Chen, Danrui Jiang, Enping Zhu, Wei Li, Peng Guo, Hongsheng Chen, Zhangquan Wang, Sen Onco Targets Ther Original Research Background: Growing evidence has indicated that interleukin-37 (IL-37) is a potential anticancer molecule that mainly plays an inhibiting role in different kinds of cancers, but data for the role of IL-37 on cell apoptosis in cancers remains rare. The present study aimed to explore the role of IL-37 in cell apoptosis in cervical cancer, and the involved apoptosis-related molecules. Methods: IL-37 was overexpressed by transfecting the pIRES2-EGFP-IL-37 plasmid in HeLa and C33A cells. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to detect the mRNA expression of IL-37, Bcl-2, Bax and Bim. Western blotting was performed to detect the protein expression of IL-37 and Bim. Cell apoptosis was detected by flow cytometry. Results: IL-37 upregulated the mRNA expression levels of Bim by 138.40% for HeLa (P<0.05) and 58.95% for C33A (P<0.05), and increased the protein expression levels of BimL by 69.10% (P<0.05) and 56.66% (P<0.05) in HeLa and C33A, respectively. Overexpression of IL-37 increased the apoptosis rates by 152.86% for HeLa (P<0.01) and 25.4% for C33A (P<0.05). Knockdown of Bim by specific siRNA interference fragments (SiBim) reduced the apoptosis rates by 36.00% for HeLa (P<0.05) and 14.66% for C33A (P<0.05). Compared with the IL-37 overexpression group, the apoptosis rate in cotransfecting the IL-37 overexpression plasmid and SiBim group decreased by approximately 31% (P<0.05) and 24.35% (P<0.05) in HeLa and C33A, respectively. Conclusion: IL-37 upregulated Bim in cervical cancer cells. Furthermore, IL-37 can promote cervical cancer cell apoptosis, but Bim knockdown decreased this promotion through IL-37. Thus, IL-37 can promote cervical cancer cell apoptosis, which involve the upregulation of Bim. Dove 2019-04-10 /pmc/articles/PMC6497894/ /pubmed/31114224 http://dx.doi.org/10.2147/OTT.S201664 Text en © 2019 Ouyang et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Ouyang, Ping An, Weifang Chen, Renhuai Zhang, He Chen, Danrui Jiang, Enping Zhu, Wei Li, Peng Guo, Hongsheng Chen, Zhangquan Wang, Sen IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title | IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title_full | IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title_fullStr | IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title_full_unstemmed | IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title_short | IL-37 promotes cell apoptosis in cervical cancer involving Bim upregulation |
title_sort | il-37 promotes cell apoptosis in cervical cancer involving bim upregulation |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6497894/ https://www.ncbi.nlm.nih.gov/pubmed/31114224 http://dx.doi.org/10.2147/OTT.S201664 |
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