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Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae

Colistin has become a last-resort antibiotic for the treatment of infections caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector. As a consequence, colistin resistance is emerging worldwide. Among the colistin resistance mechanisms, the s...

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Autores principales: Volland, Hervé, Dortet, Laurent, Bernabeu, Sandrine, Boutal, Hervé, Haenni, Marisa, Madec, Jean-Yves, Robin, Frédéric, Beyrouthy, Racha, Naas, Thierry, Simon, Stéphanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498016/
https://www.ncbi.nlm.nih.gov/pubmed/30842227
http://dx.doi.org/10.1128/JCM.01454-18
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author Volland, Hervé
Dortet, Laurent
Bernabeu, Sandrine
Boutal, Hervé
Haenni, Marisa
Madec, Jean-Yves
Robin, Frédéric
Beyrouthy, Racha
Naas, Thierry
Simon, Stéphanie
author_facet Volland, Hervé
Dortet, Laurent
Bernabeu, Sandrine
Boutal, Hervé
Haenni, Marisa
Madec, Jean-Yves
Robin, Frédéric
Beyrouthy, Racha
Naas, Thierry
Simon, Stéphanie
author_sort Volland, Hervé
collection PubMed
description Colistin has become a last-resort antibiotic for the treatment of infections caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector. As a consequence, colistin resistance is emerging worldwide. Among the colistin resistance mechanisms, the spread of the plasmid-encoded colistin resistance gene mcr-1 (mostly in Escherichia coli) is of particular concern due to its increased transferability compared to that of chromosome-encoded resistance. The early detection of MCR-1-producing bacteria is essential to prevent further spread and provide appropriate antimicrobial therapy. Lateral flow immunoassays (LFIAs) were manufactured with selected monoclonal antibodies. A collection of 177 human and 121 animal enterobacterial isolates was tested in a multicentric study. One bacterial colony grown on agar plates was suspended in extraction buffer and dispensed on the cassette. Migration was allowed for 15 min, and the results were monitored by the appearance of a specific band. The positive results showed a pink line resulting in an unambiguous interpretation. All MCR-1-producing isolates were found to be positive by the LFIA, and no false-negative results were observed. Three out of four MCR-2-producing isolates were also found to be positive. Our test does not detect MCR-3-, MCR-4-, or MCR-5-producing isolates. LFIA allows the detection of MCR-1 with 100% sensitivity and 98% specificity. This test is fast, sensitive, specific, easy to use, and cost-effective and can therefore be implemented in any microbiology laboratory worldwide. LFIA is a major tool for the rapid detection and monitoring of MCR-1 producers in humans and animals.
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spelling pubmed-64980162019-06-03 Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae Volland, Hervé Dortet, Laurent Bernabeu, Sandrine Boutal, Hervé Haenni, Marisa Madec, Jean-Yves Robin, Frédéric Beyrouthy, Racha Naas, Thierry Simon, Stéphanie J Clin Microbiol Immunoassays Colistin has become a last-resort antibiotic for the treatment of infections caused by highly drug-resistant Gram-negative bacteria. Moreover, it has been widely used in the livestock sector. As a consequence, colistin resistance is emerging worldwide. Among the colistin resistance mechanisms, the spread of the plasmid-encoded colistin resistance gene mcr-1 (mostly in Escherichia coli) is of particular concern due to its increased transferability compared to that of chromosome-encoded resistance. The early detection of MCR-1-producing bacteria is essential to prevent further spread and provide appropriate antimicrobial therapy. Lateral flow immunoassays (LFIAs) were manufactured with selected monoclonal antibodies. A collection of 177 human and 121 animal enterobacterial isolates was tested in a multicentric study. One bacterial colony grown on agar plates was suspended in extraction buffer and dispensed on the cassette. Migration was allowed for 15 min, and the results were monitored by the appearance of a specific band. The positive results showed a pink line resulting in an unambiguous interpretation. All MCR-1-producing isolates were found to be positive by the LFIA, and no false-negative results were observed. Three out of four MCR-2-producing isolates were also found to be positive. Our test does not detect MCR-3-, MCR-4-, or MCR-5-producing isolates. LFIA allows the detection of MCR-1 with 100% sensitivity and 98% specificity. This test is fast, sensitive, specific, easy to use, and cost-effective and can therefore be implemented in any microbiology laboratory worldwide. LFIA is a major tool for the rapid detection and monitoring of MCR-1 producers in humans and animals. American Society for Microbiology 2019-04-26 /pmc/articles/PMC6498016/ /pubmed/30842227 http://dx.doi.org/10.1128/JCM.01454-18 Text en Copyright © 2019 Volland et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Immunoassays
Volland, Hervé
Dortet, Laurent
Bernabeu, Sandrine
Boutal, Hervé
Haenni, Marisa
Madec, Jean-Yves
Robin, Frédéric
Beyrouthy, Racha
Naas, Thierry
Simon, Stéphanie
Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title_full Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title_fullStr Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title_full_unstemmed Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title_short Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae
title_sort development and multicentric validation of a lateral flow immunoassay for rapid detection of mcr-1-producing enterobacteriaceae
topic Immunoassays
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498016/
https://www.ncbi.nlm.nih.gov/pubmed/30842227
http://dx.doi.org/10.1128/JCM.01454-18
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