Cargando…

Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics

OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulate...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Jong Nam, Song, Jaeyong, Kim, Eun Joong, Chang, Jongsoo, Kim, Chang-Hyun, Seo, Seongwon, Chang, Moon Baek, Bae, Gui-Seck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498081/
https://www.ncbi.nlm.nih.gov/pubmed/30208688
http://dx.doi.org/10.5713/ajas.18.0489
_version_ 1783415575169466368
author Kim, Jong Nam
Song, Jaeyong
Kim, Eun Joong
Chang, Jongsoo
Kim, Chang-Hyun
Seo, Seongwon
Chang, Moon Baek
Bae, Gui-Seck
author_facet Kim, Jong Nam
Song, Jaeyong
Kim, Eun Joong
Chang, Jongsoo
Kim, Chang-Hyun
Seo, Seongwon
Chang, Moon Baek
Bae, Gui-Seck
author_sort Kim, Jong Nam
collection PubMed
description OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. RESULTS: Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p<0.05). No significant differences, however, was observed in pH and dry matter digestibility. CONCLUSION: When in vitro techniques are used to evaluate feed, the use of the ruminal fluid from fasted animals should be used with caution.
format Online
Article
Text
id pubmed-6498081
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
record_format MEDLINE/PubMed
spelling pubmed-64980812019-06-01 Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics Kim, Jong Nam Song, Jaeyong Kim, Eun Joong Chang, Jongsoo Kim, Chang-Hyun Seo, Seongwon Chang, Moon Baek Bae, Gui-Seck Asian-Australas J Anim Sci Article OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. RESULTS: Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p<0.05). No significant differences, however, was observed in pH and dry matter digestibility. CONCLUSION: When in vitro techniques are used to evaluate feed, the use of the ruminal fluid from fasted animals should be used with caution. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019-06 2018-09-13 /pmc/articles/PMC6498081/ /pubmed/30208688 http://dx.doi.org/10.5713/ajas.18.0489 Text en Copyright © 2019 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Kim, Jong Nam
Song, Jaeyong
Kim, Eun Joong
Chang, Jongsoo
Kim, Chang-Hyun
Seo, Seongwon
Chang, Moon Baek
Bae, Gui-Seck
Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title_full Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title_fullStr Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title_full_unstemmed Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title_short Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
title_sort effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498081/
https://www.ncbi.nlm.nih.gov/pubmed/30208688
http://dx.doi.org/10.5713/ajas.18.0489
work_keys_str_mv AT kimjongnam effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT songjaeyong effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT kimeunjoong effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT changjongsoo effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT kimchanghyun effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT seoseongwon effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT changmoonbaek effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics
AT baeguiseck effectsofshorttermfastingoninvivorumenmicrobiotaandinvitrorumenfermentationcharacteristics