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Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics
OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulate...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498081/ https://www.ncbi.nlm.nih.gov/pubmed/30208688 http://dx.doi.org/10.5713/ajas.18.0489 |
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author | Kim, Jong Nam Song, Jaeyong Kim, Eun Joong Chang, Jongsoo Kim, Chang-Hyun Seo, Seongwon Chang, Moon Baek Bae, Gui-Seck |
author_facet | Kim, Jong Nam Song, Jaeyong Kim, Eun Joong Chang, Jongsoo Kim, Chang-Hyun Seo, Seongwon Chang, Moon Baek Bae, Gui-Seck |
author_sort | Kim, Jong Nam |
collection | PubMed |
description | OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. RESULTS: Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p<0.05). No significant differences, however, was observed in pH and dry matter digestibility. CONCLUSION: When in vitro techniques are used to evaluate feed, the use of the ruminal fluid from fasted animals should be used with caution. |
format | Online Article Text |
id | pubmed-6498081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-64980812019-06-01 Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics Kim, Jong Nam Song, Jaeyong Kim, Eun Joong Chang, Jongsoo Kim, Chang-Hyun Seo, Seongwon Chang, Moon Baek Bae, Gui-Seck Asian-Australas J Anim Sci Article OBJECTIVE: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. METHODS: Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. RESULTS: Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p<0.05). No significant differences, however, was observed in pH and dry matter digestibility. CONCLUSION: When in vitro techniques are used to evaluate feed, the use of the ruminal fluid from fasted animals should be used with caution. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2019-06 2018-09-13 /pmc/articles/PMC6498081/ /pubmed/30208688 http://dx.doi.org/10.5713/ajas.18.0489 Text en Copyright © 2019 by Asian-Australasian Journal of Animal Sciences This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Kim, Jong Nam Song, Jaeyong Kim, Eun Joong Chang, Jongsoo Kim, Chang-Hyun Seo, Seongwon Chang, Moon Baek Bae, Gui-Seck Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title | Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title_full | Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title_fullStr | Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title_full_unstemmed | Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title_short | Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
title_sort | effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498081/ https://www.ncbi.nlm.nih.gov/pubmed/30208688 http://dx.doi.org/10.5713/ajas.18.0489 |
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