Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)

In article number 1801361, Yao Lu, Rong Fan, and co‐workers demonstrate a multiplexed antibody‐barcode‐integrated microtrough array chip, that sequentially measures protein secretions from >5000 single macrophage cells upon activation. The protein secretion dynamics classifies them into two major...

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Detalles Bibliográficos
Autores principales: Chen, Zhuo, Lu, Yao, Zhang, Kerou, Xiao, Yang, Lu, Jun, Fan, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Inside Back Cover
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498133/
http://dx.doi.org/10.1002/advs.201970055
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author Chen, Zhuo
Lu, Yao
Zhang, Kerou
Xiao, Yang
Lu, Jun
Fan, Rong
author_facet Chen, Zhuo
Lu, Yao
Zhang, Kerou
Xiao, Yang
Lu, Jun
Fan, Rong
author_sort Chen, Zhuo
collection PubMed
description In article number 1801361, Yao Lu, Rong Fan, and co‐workers demonstrate a multiplexed antibody‐barcode‐integrated microtrough array chip, that sequentially measures protein secretions from >5000 single macrophage cells upon activation. The protein secretion dynamics classifies them into two major activation states dependent on the basal state of each cell, showing a cell‐intrinsic heterogeneous response in a phenotypically homogeneous cell population. [Image: see text]
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spelling pubmed-64981332019-05-07 Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019) Chen, Zhuo Lu, Yao Zhang, Kerou Xiao, Yang Lu, Jun Fan, Rong Adv Sci (Weinh) Inside Back Cover In article number 1801361, Yao Lu, Rong Fan, and co‐workers demonstrate a multiplexed antibody‐barcode‐integrated microtrough array chip, that sequentially measures protein secretions from >5000 single macrophage cells upon activation. The protein secretion dynamics classifies them into two major activation states dependent on the basal state of each cell, showing a cell‐intrinsic heterogeneous response in a phenotypically homogeneous cell population. [Image: see text] John Wiley and Sons Inc. 2019-05-03 /pmc/articles/PMC6498133/ http://dx.doi.org/10.1002/advs.201970055 Text en © 2019 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Inside Back Cover
Chen, Zhuo
Lu, Yao
Zhang, Kerou
Xiao, Yang
Lu, Jun
Fan, Rong
Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title_full Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title_fullStr Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title_full_unstemmed Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title_short Single‐Cell Cytokine Assays: Multiplexed, Sequential Secretion Analysis of the Same Single Cells Reveals Distinct Effector Response Dynamics Dependent on the Initial Basal State (Adv. Sci. 9/2019)
title_sort single‐cell cytokine assays: multiplexed, sequential secretion analysis of the same single cells reveals distinct effector response dynamics dependent on the initial basal state (adv. sci. 9/2019)
topic Inside Back Cover
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498133/
http://dx.doi.org/10.1002/advs.201970055
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