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Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization
Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time‐dependent manner in m...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498523/ https://www.ncbi.nlm.nih.gov/pubmed/30746892 http://dx.doi.org/10.1002/vms3.151 |
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author | Ferrão, Juliana S. P. Bonfim Neto, Antenor P. da Fonseca, Vanessa U. Sousa, Liza M.M.de C. Papa, Paula de C. |
author_facet | Ferrão, Juliana S. P. Bonfim Neto, Antenor P. da Fonseca, Vanessa U. Sousa, Liza M.M.de C. Papa, Paula de C. |
author_sort | Ferrão, Juliana S. P. |
collection | PubMed |
description | Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time‐dependent manner in mouse hindlimb (HL) after resection of the inguinal lymph node. Lymphatic vascular density (Vv) and length (Lv) were evaluated by stereology after immunohistochemistry. The control Group (CG) was not manipulated but received saline instead of VEGFC treatment. The surgery Group (SG) had the left inguinal lymph node resected but did not received VEGFC treatment. VEGFC Treated Group (TG) had the node resected and received VEGFC treatment. VEGFC and VEGFR3 local expression were assessed by qPCR. There was an effect of time over Vv and Lv in the SG and significant difference between CG and SG in the regions studied (proximal, medium and distal regions) of the left HL (LHL). The Lv showed significant difference between CG and SG only in the medium region. The Vv and the Lv for TG were higher than the other groups. VEGFC and VEGFR3 gene expression presented time effect in all regions of the LHL for SG and TG. Both VEGFC and VEGFR3 gene expression presented significant difference between CG and SG, between SG and TG and between CG and TG. This study showed significant decrease in lymphatic vascularization in the left hindlimb of mice after surgical removal of the inguinal lymph node and adjacent lymphatic vessels. Exogenous VEGFC could recover lymphatic vascularization through stimulating neolymphangiogenesis. |
format | Online Article Text |
id | pubmed-6498523 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-64985232019-05-07 Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization Ferrão, Juliana S. P. Bonfim Neto, Antenor P. da Fonseca, Vanessa U. Sousa, Liza M.M.de C. Papa, Paula de C. Vet Med Sci Original Articles Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time‐dependent manner in mouse hindlimb (HL) after resection of the inguinal lymph node. Lymphatic vascular density (Vv) and length (Lv) were evaluated by stereology after immunohistochemistry. The control Group (CG) was not manipulated but received saline instead of VEGFC treatment. The surgery Group (SG) had the left inguinal lymph node resected but did not received VEGFC treatment. VEGFC Treated Group (TG) had the node resected and received VEGFC treatment. VEGFC and VEGFR3 local expression were assessed by qPCR. There was an effect of time over Vv and Lv in the SG and significant difference between CG and SG in the regions studied (proximal, medium and distal regions) of the left HL (LHL). The Lv showed significant difference between CG and SG only in the medium region. The Vv and the Lv for TG were higher than the other groups. VEGFC and VEGFR3 gene expression presented time effect in all regions of the LHL for SG and TG. Both VEGFC and VEGFR3 gene expression presented significant difference between CG and SG, between SG and TG and between CG and TG. This study showed significant decrease in lymphatic vascularization in the left hindlimb of mice after surgical removal of the inguinal lymph node and adjacent lymphatic vessels. Exogenous VEGFC could recover lymphatic vascularization through stimulating neolymphangiogenesis. John Wiley and Sons Inc. 2019-02-11 /pmc/articles/PMC6498523/ /pubmed/30746892 http://dx.doi.org/10.1002/vms3.151 Text en © 2019 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ferrão, Juliana S. P. Bonfim Neto, Antenor P. da Fonseca, Vanessa U. Sousa, Liza M.M.de C. Papa, Paula de C. Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title | Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title_full | Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title_fullStr | Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title_full_unstemmed | Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title_short | Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization |
title_sort | vascular endothelial growth factor c treatment for mouse hind limb lymphatic revascularization |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498523/ https://www.ncbi.nlm.nih.gov/pubmed/30746892 http://dx.doi.org/10.1002/vms3.151 |
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