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Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context

We report a clinical strain of Enterobacter cloacae, PIMB10EC27, isolated in Vietnam in 2010 that was resistant to 21 of 26 tested antibiotics, including carbapenems (MICs >64 µg/mL) and colistin (MIC >128 µg/mL). The complete genome of strain PIMB10EC27 was sequenced by PacBio RSII and the Il...

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Autores principales: Le-Ha, Tam-Duong, Le, Lien, Le-Vo, Hong-Ngoc, Anda, Mizue, Motooka, Daisuke, Nakamura, Shota, Tran, Linh Khanh, Tran, Phuong Thi-Bich, Iida, Tetsuya, Cao, Van
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498974/
https://www.ncbi.nlm.nih.gov/pubmed/31118694
http://dx.doi.org/10.2147/IDR.S194495
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author Le-Ha, Tam-Duong
Le, Lien
Le-Vo, Hong-Ngoc
Anda, Mizue
Motooka, Daisuke
Nakamura, Shota
Tran, Linh Khanh
Tran, Phuong Thi-Bich
Iida, Tetsuya
Cao, Van
author_facet Le-Ha, Tam-Duong
Le, Lien
Le-Vo, Hong-Ngoc
Anda, Mizue
Motooka, Daisuke
Nakamura, Shota
Tran, Linh Khanh
Tran, Phuong Thi-Bich
Iida, Tetsuya
Cao, Van
author_sort Le-Ha, Tam-Duong
collection PubMed
description We report a clinical strain of Enterobacter cloacae, PIMB10EC27, isolated in Vietnam in 2010 that was resistant to 21 of 26 tested antibiotics, including carbapenems (MICs >64 µg/mL) and colistin (MIC >128 µg/mL). The complete genome of strain PIMB10EC27 was sequenced by PacBio RSII and the Illumina Miseq system. Whole-genome analysis revealed that PIMB10EC27 contains a chromosome of the ST513 group (PIMBEC27, length 5,272,177 bp) and two plasmids, pEC27-1 of the IncX3 group (length 62,470 bp) and pEC27-2 of the IncHI1 group (length 84,602 bp). It also revealed that strain PIMB10EC27 carries 15 genes that confer resistance to at least 10 antibiotic groups. Particularly, the insertion of ISKpn19 and Tn6901 into the genomic context of bla(NDM-1) was first identified and described. In another context, amino acid mutations G273D in PmrB and F515S in PmrC were first identified on the chromosome of PIMB10EC27, which may confer resistance to colistin in this strain.
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spelling pubmed-64989742019-05-22 Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context Le-Ha, Tam-Duong Le, Lien Le-Vo, Hong-Ngoc Anda, Mizue Motooka, Daisuke Nakamura, Shota Tran, Linh Khanh Tran, Phuong Thi-Bich Iida, Tetsuya Cao, Van Infect Drug Resist Short Report We report a clinical strain of Enterobacter cloacae, PIMB10EC27, isolated in Vietnam in 2010 that was resistant to 21 of 26 tested antibiotics, including carbapenems (MICs >64 µg/mL) and colistin (MIC >128 µg/mL). The complete genome of strain PIMB10EC27 was sequenced by PacBio RSII and the Illumina Miseq system. Whole-genome analysis revealed that PIMB10EC27 contains a chromosome of the ST513 group (PIMBEC27, length 5,272,177 bp) and two plasmids, pEC27-1 of the IncX3 group (length 62,470 bp) and pEC27-2 of the IncHI1 group (length 84,602 bp). It also revealed that strain PIMB10EC27 carries 15 genes that confer resistance to at least 10 antibiotic groups. Particularly, the insertion of ISKpn19 and Tn6901 into the genomic context of bla(NDM-1) was first identified and described. In another context, amino acid mutations G273D in PmrB and F515S in PmrC were first identified on the chromosome of PIMB10EC27, which may confer resistance to colistin in this strain. Dove 2019-04-03 /pmc/articles/PMC6498974/ /pubmed/31118694 http://dx.doi.org/10.2147/IDR.S194495 Text en © 2019 Le-Ha et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Short Report
Le-Ha, Tam-Duong
Le, Lien
Le-Vo, Hong-Ngoc
Anda, Mizue
Motooka, Daisuke
Nakamura, Shota
Tran, Linh Khanh
Tran, Phuong Thi-Bich
Iida, Tetsuya
Cao, Van
Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title_full Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title_fullStr Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title_full_unstemmed Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title_short Characterization of a carbapenem- and colistin-resistant Enterobacter cloacae carrying Tn6901 in bla(NDM-1) genomic context
title_sort characterization of a carbapenem- and colistin-resistant enterobacter cloacae carrying tn6901 in bla(ndm-1) genomic context
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498974/
https://www.ncbi.nlm.nih.gov/pubmed/31118694
http://dx.doi.org/10.2147/IDR.S194495
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