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In vitro analysis of RNA polymerase II elongation complex dynamics

RNA polymerase II elongation complexes (ECs) were assembled from nuclear extract on immobilized DNA templates and analyzed by quantitative mass spectrometry. Time-course experiments showed that initiation factor TFIIF can remain bound to early ECs, while levels of core elongation factors Spt4–Spt5,...

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Detalles Bibliográficos
Autores principales:	Joo, Yoo Jin, Ficarro, Scott B., Chun, Yujin, Marto, Jarrod A., Buratowski, Stephen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Cold Spring Harbor Laboratory Press 2019
Materias:	Research Paper
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499329/ https://www.ncbi.nlm.nih.gov/pubmed/30846429 http://dx.doi.org/10.1101/gad.324202.119

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