Split-QF System for Fine-Tuned Transgene Expression in Drosophila

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-Q...

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Detalles Bibliográficos
Autores principales: Riabinina, Olena, Vernon, Samuel W., Dickson, Barry J., Baines, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499530/
https://www.ncbi.nlm.nih.gov/pubmed/30862621
http://dx.doi.org/10.1534/genetics.119.302034
Descripción
Sumario:The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.

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