Split-QF System for Fine-Tuned Transgene Expression in Drosophila

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-Q...

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Autores principales: Riabinina, Olena, Vernon, Samuel W., Dickson, Barry J., Baines, Richard A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2019
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499530/
https://www.ncbi.nlm.nih.gov/pubmed/30862621
http://dx.doi.org/10.1534/genetics.119.302034
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author Riabinina, Olena
Vernon, Samuel W.
Dickson, Barry J.
Baines, Richard A.
author_facet Riabinina, Olena
Vernon, Samuel W.
Dickson, Barry J.
Baines, Richard A.
author_sort Riabinina, Olena
collection PubMed
description The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms.
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spelling pubmed-64995302020-05-01 Split-QF System for Fine-Tuned Transgene Expression in Drosophila Riabinina, Olena Vernon, Samuel W. Dickson, Barry J. Baines, Richard A. Genetics Investigations The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila, is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila, and in other organisms. Genetics Society of America 2019-05 2019-03-12 /pmc/articles/PMC6499530/ /pubmed/30862621 http://dx.doi.org/10.1534/genetics.119.302034 Text en Copyright © 2019 Riabinina et al. Available freely online through the author-supported open access option. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Riabinina, Olena
Vernon, Samuel W.
Dickson, Barry J.
Baines, Richard A.
Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title_full Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title_fullStr Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title_full_unstemmed Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title_short Split-QF System for Fine-Tuned Transgene Expression in Drosophila
title_sort split-qf system for fine-tuned transgene expression in drosophila
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6499530/
https://www.ncbi.nlm.nih.gov/pubmed/30862621
http://dx.doi.org/10.1534/genetics.119.302034
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AT vernonsamuelw splitqfsystemforfinetunedtransgeneexpressionindrosophila
AT dicksonbarryj splitqfsystemforfinetunedtransgeneexpressionindrosophila
AT bainesricharda splitqfsystemforfinetunedtransgeneexpressionindrosophila

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